Acta Med. 2019, 62: 30-34

https://doi.org/10.14712/18059694.2019.43

Cytotoxic Effects of Pistacia Atlantica (Baneh) Fruit Extract on Human KB Cancer Cell Line

Zohreh Jaafari-Ashkvandia, Saeed Yousefi Shirazib, Somayeh Rezaeifardc, Azadeh Hamedid, Nasrollah Erfanie

aDepartment of Oral & Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
bSchool of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
cShiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
dDepartment of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
eDepartment of Immunology and Shiraz Institute for Cancer Researches, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Received September 25, 2018
Accepted January 26, 2019

Plants with anticancer properties are considered as cancer preventive and treatment sources, due to their some biological effects. Apoptosis induction and anti-proliferative effects of Baneh extract on various cancer cell lines have been reported. Hence, this study was designed to evaluate the cytotoxic effects of this fruit on KB and human gingival fibroblast cell lines (HGF). KB and HGF cells were treated with various concentrations of ethanolic Baneh extract and cisplatin as positive control. Cytotoxic activity and apoptosis induction were investigated using WST-1 and Annexin V assays. Data were analyzed using ANOVA and student’s t-tests. IC50 after 24 and 48 hours treatment were respectively 2.6 and 1 mg/mL for KB cell line, and 1.5 and 1.6 mg/mL for HGF cell. During 48 hours Baneh extract induced apoptosis without significant necrosis, in a time- and dose-dependent manner. The induction of apoptosis in KB cells was significantly higher than HGF. It seems that ethanolic extract of Baneh contains compounds that can suppress KB cell growth through the induction of apoptosis. Within 48 hours, less cytotoxic effects were observed on normal fibroblast cells; therefore, it might be a potential anticancer agent.

Funding

This research program was funded by Vice-chancellery of Shiraz University of Medical Sciences (Grant #8895148).

References

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