Acta Med. 2016, 59: 107-112

Histological Evaluation of Decellularized Skeletal Muscle Tissue Using Two Different Decellularization Agents

Hana Hrebíková, Magda Voborníková, Milada Hetešová, Jaroslav Mokrý

Department of Histology and Embryology, Charles University, Medical Faculty, Hradec Králové, Czech Republic

Received August 3, 2016
Accepted October 19, 2016

The aim of the present study was to determine effect of two decellularized agents, sodium dodecyl sulphate (SDS) and Triton X-100, to the skeletal muscle tissue. Final scaffold was evaluated by several histological techniques to analyse preservation of essential structures including collagen and elastic fibres, basement membranes, glycosaminoglycans and also to confirm elimination of nuclear and cytoplasmic components which are redundant in effectively prepared decellularized scaffolds. Comparison of tissue scaffolds processed with different detergents proved that SDS is superior to Triton X-100 as it can effectively decellularize muscle tissue.


This work was supported by the Grant Agency of the Czech Republic 15-09161S, Grant of the Charles University No. SVV-2016-260278 and PRVOUK P37/06.


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