Acta Med. 2009, 52: 69-72

Tissue Specific Sensitivity of Mitochondrial Permeability Transition Pore to Ca2+ Ions

René Endlichera, Pavla Křivákováa, Halka Lotkováa, Marie Milerováb, Zdeněk Drahotaa,b, Zuzana Červinkováa

aCharles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Physiology, Hradec Králové, Czech Republic
bAcademy of Sciences of the Czech Republic, Institute of Physiology, Prague, Czech Republic

Received February 1, 2009
Accepted June 1, 2009

Ca2+-induced opening of the mitochondrial permeability transition pore (MPTP) is involved in induction of apoptotic and necrotic processes. We studied sensitivity of MPTP to calcium using the model of Ca2+-induced, cyclosporine A-sensitive mitochondrial swelling. Presented data indicate that the extent of mitochondrial swelling (dA520/4 min) induced by addition of 25 μM Ca2+ is seven-fold higher in liver than in heart mitochondria (0.564 ± 0.08/0.077± 0.01). The extent of swelling induced by 100 μM Ca2+ was in liver tree times higher than in heart mitochondria (0.508±0.05/ 0.173±0.02). Cyclosporine A sensitivity showed that opening of the MPTP is involved. We may thus conclude that especially at low Ca2+ concentration heart mitochondria are more resistant to damaging effect of Ca2+ than liver mitochondria. These finding thus support hypothesis that there exist tissue specific strategies of cell protection against induction of the apoptotic and necrotic processes.


This work was supported by the Ministry of Education Research Project MSM VZ 0021620820 and Institutional project AVOZ 501509.


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