Acta Med. 2007, 50: 171-176
In Vitro Antiproliferative Effects of Sulforaphane on Human Colon Cancer Cell Line SW620
The isothiocyanate sulforaphane (SF) has been reported to possess chemopreventive efficiency towards various malignancies including colon cancer. Here, we investigated the antiproliferative and pro-apoptotic effects of SF on colon cancer cell line SW620. We found that SF at concentrations of 10–50 μM inhibits cell viability and proliferation of SW620 cells in a time- and dose-dependent manner, with IC50 being 26 μM (24 h), 24.4 μM (48 h) and 18 μM (72 h). Also, in the same cells SF caused DNA damage and chromatin condensation after 24 h and 48 h as revealed by phospho-H2A.X western blot analysis and DAPI staining of nuclei. These changes were accompanied by the elevated activity of caspase 3, although after 20 μM SF concentration only. Together, these results indicate that SF suppresses growth of human metastacic colonocytes and induces apoptotic cell death.
Keywords
SW620 cells, Caspase 3, Sulforaphane, Apoptosis, Colon cancer, Proliferation, In vitro.
Funding
This work has been supported by Ministry of Education Research Project MSM 0021620820.
References
Copyright
Published by the Karolinum Press. For permission to use please write to actamedica@lfhk.cuni.cz.
