Acta Med. 2007, 50: 203-206

https://doi.org/10.14712/18059694.2017.83

Evaluation of Potency of Known Oximes (Pralidoxime, Trimedoxime, HI-6, Methoxime, Obidoxime) to in vitro Reactivate Acetylcholinesterase Inhibited by Pesticides (Chlorpyrifos and Methylchlorpyrifos and Nerve Agent (Russian VX)

Kamil Musíleka,b, Kamil Kučab,c, Daniel Junb,c

aCharles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control, Hradec Králové, Czech Republic
bUniversity of Defence, Faculty of Military Health Sciences, Department of Toxicology, Hradec Králové, Czech Republic
cUniversity of Defence, Faculty of Military Health Sciences, Centre of Advanced Studies, Hradec Králové, Czech Republic

Received March 1, 2007
Accepted July 1, 2007

Nerve agents and pesticides belong to the group of organophosphates. They are able to inhibit irreversibly the enzyme acetylcholinesterase (AChE). Acetylcholinesterase reactivators were designed for the treatment of nerve agent intoxications. Their potency to reactivate pesticide-inhibited AChE was many times evaluated. In this study, five commonly used AChE reactivators (pralidoxime, methoxime, HI-6, obidoxime, trimedoxime) for the reactivation of AChE inhibited by two pesticides (chlorpyrifos and methylchlorpyrifos) were used. Russian VX (nerve agent) as a member of nerve agents’ family was taken for comparison. Obtained results show that oximes developed against nerve agent intoxication are less effective for intoxication with organophosphorus pesticides. Especially, methylchlorpyrifos-inhibited AChE was found to be poorly reactivated by the compounds used.

Funding

The work was supported by the grant of Grant Agency of Ministry of Education, Youth and Sports (Czech Republic) – project No. ME865.

References

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