Lithium Microdialysis and Its Use for Monitoring of Stomach and Colon Submucosal Blood Perfusion – A Pilot Study Using Ischemic Preconditioning in Rats

Cibiček, Norbert; Mičuda, Stanislav; Chládek, Jaroslav; Živný, Pavel; Zadák, Zdeněk; Čermáková, Eva; Palička, Vladimír

doi:10.14712/18059694.2017.137

Acta Medica > Archive > 2006, Vol. 49 > Issue 4 > Lithium Microdialysis and Its Use for…

Acta Med. 2006, 49: 227-231

https://doi.org/10.14712/18059694.2017.137

Lithium Microdialysis and Its Use for Monitoring of Stomach and Colon Submucosal Blood Perfusion – A Pilot Study Using Ischemic Preconditioning in Rats

Norbert Cibiček^a, Stanislav Mičuda^b, Jaroslav Chládek^b, Pavel Živný^a, Zdeněk Zadák^c, Eva Čermáková^d, Vladimír Palička^a

^aCharles University in Prague, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Institute for Clinical Biochemistry and Diagnostics, Hradec Králové, Czech Republic
^bCharles University in Prague, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Department of Pharmacology, Hradec Králové, Czech Republic
^cCharles University in Prague, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Department of Gerontology and Metabolism, Research Laboratory, Hradec Králové, Czech Republic
^dCharles University in Prague, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Department of Biophysics and Biostatistics, Hradec Králové, Czech Republic

Received August 1, 2006
Accepted September 1, 2006

During shock, exposure of gut to ischemia determines patient’s survival. Ischemic preconditioning (ISP) elevates nitric oxide and blood perfusion, whereby it protects organs against subsequent severe ischemia/reperfusion. Using appropriate flow marker, microdialysis may serve to monitor interstitial microcirculation. Hence, our aim was to test the reliability of lithium as a flow marker (lithium microdialysis, LM) on an ISP model. Rats were divided into three groups. Two (ischemic and preconditioned) groups underwent 30 min celiac artery occlusion (CAO) with 2.5 h reperfusion. 25 min before CAO, the latter experienced 5 min ischemia. Sham–operated animals served as controls. LM in stomach and colon submucosa, serum nitric oxide, hepatic and pancreatic enzymes were measured. In stomach, LM indicated a decrease in blood perfusion evoked by CAO (p<0.01) in both experimental groups. During reperfusion, the ischemic animals showed a restoration of microcirculation, unlike the preconditioned ones, whose blood perfusion failed to regenerate (p<0.001). For any group, LM showed no microcirculation modification in colon. Serum analytes remained unchanged. We conclude that LM appears to be a potentially suitable indicator of gastrointestinal interstitial microcirculation. However, we failed to demonstrate any beneficial effect of ISP on pancreas, systemic nitric oxide and local/remote microcirculation within studied organs.

Keywords

Microdialysis, Microcirculation, Gastrointestinal tract, Ischemic preconditioning.

Funding

This work was principally supported by a Research Project from the Czech Ministry of Foreign Commerce (MZO 00179906), and in part also by a grant from the Czech Ministry of Education (MŠMT COST B25).