Acta Med. 2006, 49: 27-33

https://doi.org/10.14712/18059694.2017.106

Mesenchymal Stem Cells Isolated from the Human Bone Marrow: Cultivation, Phenotypic Analysis and Changes in Proliferation Kinetics

Tomáš Soukupa, Jaroslav Mokrýa, Jana Karbanováa, Robert Pytlíkb, Petr Suchomelc, Lenka Kučerovád

aCharles University in Prague, Faculty of Medicine in Hradec Králové, Department of Histology and Embryology, Hradec Králové, Czech Republic
bTeaching Hospital, 1st Medical Faculty in Prague, 1st Department of Medicine, Prague, Czech Republic
cHospital Liberec, Department of Neurosurgery, Liberec, Czech Republic
dTeaching Hospital, 1st Medical Faculty in Prague, Department of Clinical Genetics, Prague, Czech Republic

Received September 1, 2005
Accepted March 1, 2006

References

1. Arai F, Ohneda O, Miyamoto T, et al. Mesenchymal stem cells in perichondrium express activated leukocyte cell adhesion molecule and participate in bone marrow formation. J Exp Med 2002; 195(12):1549–1563. <https://doi.org/10.1084/jem.20011700> <PubMed>
2. Banfi A, Muraglia A, Dozin B, et al. Proliferation kinetics and differentiation potential of ex vivo expanded human bone marrow stromal cells: Implications for their use in cell therapy. Exp Hematol 2000; 28(6):707–15. <https://doi.org/10.1016/S0301-472X(00)00160-0>
3. Bruder SP, Jaiswal N, Haynesworth SE. Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. J Cell Biochem 1997; 64(2): 278–94. <https://doi.org/10.1002/(SICI)1097-4644(199702)64:2<278::AID-JCB11>3.0.CO;2-F>
4. Colter DC, Class R, DiGirolamo CM, et al. Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow. Proc Natl Acad Sci USA 2000; 97:3213–18. <https://doi.org/10.1073/pnas.97.7.3213>
5. Doiguchi Y, Tsukazaki T, Tomonaga T, et al. Establishment of a clonal human mesenchymal cell line that retains multilineage differentiaton capacity from a spinal hamartoma. Cell Tissue Res 2004; 317(3):237–246. <https://doi.org/10.1007/s00441-004-0942-2>
6. Fridenshtein A. Stromal bone marrow cells and the hematopoietic microenvironment. Arkh Patol. 1982; 44(10):3–11.
7. Gronthos S, Graves S, Ohta S, Simmons P. The STRO-1 + fraction of adult human bone marrow contains the osteogenic precursors. Blood 1994; 84:4164–4173.
8. Gronthos S, Mankani M, Brahim J et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA 2000; 97:13625–13630. <https://doi.org/10.1073/pnas.240309797> <PubMed>
9. Haynesworth SE, Barber MA, Caplan IA. Cell surface antigens on human marrow- derived mesenchymal cells are detected by monoclonal antibodies. Bone 1992; 13:69–80. <https://doi.org/10.1016/8756-3282(92)90363-2>
10. Huss R, Moosmann S. The co-expression of CD117 (c-kit) and osteocalcin in activated bone marrow stem cells in different diseases. Br J Haematol. 2002; 118(1):305–12. <https://doi.org/10.1046/j.1365-2141.2002.03587.x>
11. Jiang Y, Jahagirdar BN, Reinhardt L, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 2002; 418(4):41–49 + Supplementary Information www.nature.com/nature. <https://doi.org/10.1038/nature00870>
12. Minguel JJ, Erices A, Conget P. Mesenchymal stem cells. Exp Biol Med 2001; 226:507–20. <https://doi.org/10.1177/153537020122600603>
13. Pereira RF, Halford KW, O’Hara MD, et al. Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage and lung in irradiated mice. Proc Natl Acad Sci USA 1995; 92:4857–61. <https://doi.org/10.1073/pnas.92.11.4857> <PubMed>
14. Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999; 284:143–7. <https://doi.org/10.1126/science.284.5411.143>
15. Reyes M, Lund T, Lenvik T, Aguiar D, Koodie L, Verfaillie CM. Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells. Blood 2001; 98(9):2615–2625. <https://doi.org/10.1182/blood.V98.9.2615>
16. Tsai MS, Lee JL, Chang YJ, and Hwang SM. Isolation of human multipotent mesenchymal cells from second-trimester amnioti fluid using a novel two-stage culture protocol. Hum Reprod 2004; 19(6):1450–1456. <https://doi.org/10.1093/humrep/deh279>
17. Zuk PA, Zhu M, Ashjian P et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 2002; 13(12):4279–4295. <https://doi.org/10.1091/mbc.e02-02-0105>
front cover

ISSN 1211-4286 (Print) ISSN 1805-9694 (Online)

Open access journal

Archive