Acta Med. 2006, 49: 27-33

Mesenchymal Stem Cells Isolated from the Human Bone Marrow: Cultivation, Phenotypic Analysis and Changes in Proliferation Kinetics

Tomáš Soukupa, Jaroslav Mokrýa, Jana Karbanováa, Robert Pytlíkb, Petr Suchomelc, Lenka Kučerovád

aCharles University in Prague, Faculty of Medicine in Hradec Králové, Department of Histology and Embryology, Hradec Králové, Czech Republic
bTeaching Hospital, 1st Medical Faculty in Prague, 1st Department of Medicine, Prague, Czech Republic
cHospital Liberec, Department of Neurosurgery, Liberec, Czech Republic
dTeaching Hospital, 1st Medical Faculty in Prague, Department of Clinical Genetics, Prague, Czech Republic

Received September 1, 2005
Accepted March 1, 2006

Mesenchymal Stem Cells (MSCs) are rare elements living in various organs (e.g., bone marrow), able to differentiate into specialized tissues, such as bone, cartilage, tendon, and myocardium. Since the first description of MSCs by Fridenshtein, several investigators have shown that these cells can also differentiate into chondrocytes, adipocytes, and, at least, in rodents into skeletal myoblasts. Later on, more primitive progenitor cells were characterized, able to give rise not only to limb-bud mesoderm, but also to cells of visceral mesoderm. Those cells were named mesodermal progenitor cells (MPCs). The aim of our study was to characterize and compare the biological properties and spontaneous differentiation potential of two different cell types (MSCs and MPCs) isolated from the human vertebral body bone marrow. The results of our experiments proved that the MPCs can be expanded beyond Hayflick’s limit and differed from MSCs in morphology, biological and phenotypic characteristics. Because of their high proliferative and differentiation potential, MPCs can become more attractive source of adult stem cells for therapeutic purposes.


Supported by grant No. 840 23/2005 from Medical Faculty in Hradec Králové and by grant No. ND 7448/3 from IGA MZ.


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