Acta Med. 2005, 48: 29-34

Membrane Blebbing in Cancer Cells Treated with Various Apoptotic Inducers

Emil Rudolf, Miroslav Červinka

Charles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biology and Genetics, Hradec Králové, Czech Republic

Received January 1, 2005
Accepted March 1, 2005

The dynamics of cell morphology, in particular membrane blebbing, was studied after induction of apoptosis by etoposide or camptothecin in four human stabilized cell lines (Hep2, Bowes, HT-29 and HL-60). Time lapse videomicroscopy and F-actin staining revealed various dynamic parameters of this process including its duration, maximal extent, stages and final endpoints in individual cells. Although generally occurring in predictable patterns, our results indicate a relatively significant variability both in appearance and temporal organization of blebbing not only between different cell lines but also within them.


This work was supported by Ministry of Education Czech Republic Research Project MSM 0021620820.


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