The Cellular Uptake of Sensitizers Bound to Cyclodextrin Carriers

Kolářová, Hana; Huf, Martin; Maceček, Jaroslav; Nevřelová, Pavla; Tomečka, Marek; Bajgar, Robert; Mosinger, Jiří; Strnad, Miroslav

doi:10.14712/18059694.2018.114

Acta Medica > Archive > 2004, Vol. 47 > Issue 4 > The Cellular Uptake of Sensitizers Bound…

Acta Med. 2004, 47: 313-315

https://doi.org/10.14712/18059694.2018.114

The Cellular Uptake of Sensitizers Bound to Cyclodextrin Carriers

Hana Kolářová^a, Martin Huf^a, Jaroslav Maceček^a, Pavla Nevřelová^a, Marek Tomečka^a, Robert Bajgar^a, Jiří Mosinger^b, Miroslav Strnad^a

^aPalacky University Olomouc, Faculty of Medicine, Department of Biophysics, Laboratory of Growth Regulators, Olomouc, Czech Republic
^bCharles University in Prague, Faculty of Science, Department of Inorganic Chemistry, Prague, Czech Republic

Photodynamic therapy of cancer uses the interaction of sensitizers and light to destroy cancer cells. In this study we tested the cellular uptake of meso-tetrakis(4-sulfonatophenyl)porphine (TPPS4) and its complex PdTPPS4 in the presence or absence of 2–hydroxypropyl-cyclodextrins (hpCDs) on G361 human melanoma cells. Self-fluorescence in G361 cells were measured by Perkin-Elmer LS50B luminometer equipped with well plate reader accessory. Morphological changes in cells have been evaluated using inversion fluorescent microscope Olympus IX 70 and image analysis. The uptake of the sensitizer PdTPPS4 at the given time interval from 1 to 48 hours is markedly higher than the uptake of TPPS4. The highest uptake was found for sensitizer PdTPPS4 in combination with hpβCD. TPPS4 and PdTPPS4 especially in the supramolecular complex with nontoxic cyclodextrin carriers represent efficient sensitizers for photodynamic therapy in vitro on G361 cells.