Gains and Losses of HLA Class II (DR) and CD4 in Atypical Hyperplasia, Carcinoma in situ and Infiltrating Ductal Carcinoma of the Breast

Tamiolakis, Demetrio; Venizelos, Ioannis; Lambropoulou, Maria; Jivannakis, Theodoros; Seliniotaki, Evagelia; Tsikouras, Panagiotis; Limberis, Vasilios; Tsalkidis, Angelos; Papadopoulos, Nikolas

doi:10.14712/18059694.2018.101

Acta Medica > Archive > 2004, Vol. 47 > Issue 4 > Gains and Losses of HLA Class II (DR)…

Acta Med. 2004, 47: 257-262

https://doi.org/10.14712/18059694.2018.101

Gains and Losses of HLA Class II (DR) and CD4 in Atypical Hyperplasia, Carcinoma in situ and Infiltrating Ductal Carcinoma of the Breast

Demetrio Tamiolakis^a, Ioannis Venizelos^b, Maria Lambropoulou^c, Theodoros Jivannakis^c, Evagelia Seliniotaki^a, Panagiotis Tsikouras^d, Vasilios Limberis^d, Angelos Tsalkidis^e, Nikolas Papadopoulos^e

^aGeneral Hospital of Chania, Department of Cytology, Chania, Greece
^bIppokration Hospital of Salonica, Department of Pathology, Greece
^cGeneral Hospital of Drama, Department of Pathology, Greece
^dDemocritus University of Thrace, Department of Obstetrics and Gynecology, Alexandroupolis, Greece
^eDemocritus University of Thrace, Department of Histology – Embryology, Alexandroupolis, Greece

Received May 1, 2004
Accepted July 1, 2004

Aim: Breast cancer is a frequent cause of death among women with gynaecologic malignancies despite the introduction of combination chemotherapy. There is therefore a need for new therapeutic strategies for patients with breast cancer, such as cellular immunotherapy. In this immunohistochemical study we analyzed the epithelial expression of major histocompatibility complex (MHC) class II (HLA-DR) on atypical and malignant primary mammary epithelial cells, as well as the magnitude of the stromal T lymphocytes (T4 subset) at the tumor site. Experimental design: The study was carried out retrospectively in tumor tissue from 82 patients with mammary lesions (31 cases of atypical ductal hyperplasia -ADH-, 12 cases of ductal carcinoma in situ –DCIS- and 39 cases of infiltrating ductal carcinoma not otherwise specified -IDC-NOS). Medullary carcinomas were not included in our investigation. Material used had been formalin fixed and paraffin embedded. Results: HLA class II (DR) was expressed in 20 of 31 ADHs (64.5%), in 4 of 12 DCISs (33.3%), and in 10 of 39 IDC-NOSs (25.6%). CD4 was expressed in 9 of 31 ADHs (29%), in 5 of 12 DCISs (42%), and in 26 of 39 IDCNOSs (67%). Conclusions: The results showed decreased epithelial expression of HLA class II (DR) and increased stromal expression of CD4, as the lesion progressed to malignancy. Gradual loss of epithelial HLA class II expression might be a manifestation of cellular differentiation from the atypical form versus the malignant one, signaling simultaneously a selective effect on the response capacity of the immune system.