The Repair of DNA Damage Induced in Human Peripheral Lymphocytes with Styrene Oxide

Köhlerová, Renata; Štětina, Rudolf

doi:10.14712/18059694.2019.14

Acta Medica > Archive > 2003, Vol. 46 > Issue 3 > The Repair of DNA Damage Induced in…

Acta Med. 2003, 46: 95-100

https://doi.org/10.14712/18059694.2019.14

The Repair of DNA Damage Induced in Human Peripheral Lymphocytes with Styrene Oxide

Renata Köhlerová^a, Rudolf Štětina^b

^aCharles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biochemistry, Hradec Králové, Czech Republic
^bPurkyně Military Medical Academy in Hradec Králové, Department of Toxicology, Hradec Králové, Czech Republic

Received January 1, 2003
Accepted May 1, 2003

Isolated human peripheral lymphocytes were treated in vitro with styrene-7, 8–oxide (SO) and the kinetics of the repair of induced DNA damage was assessed by comet assay during further incubation of lymphocytes. Using a modified assay we measured simultaneously the number of single strand breaks in DNA (SSBs) and the sites sensitive to endonuclease III (endo III) that most probably represent abasic sites in DNA molecules. SO induced DNA damage in a dose–dependent manner and both SSBs and endo III sites were removed from the DNA by a repair process with a half time about 2–4 hours. The damage was repaired completely within 12 hours after the treatment.