Acta Med. 2000, 43: 83-89

The Dynamics of the Hexavalent Chromium Induced Apoptotic Patterns in vitro

Emil Rudolf, Jan Peychl, Miroslav Červinka

Charles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biology and Genetics, Hradec Králové, Czech Republic

Received March 1, 2000
Accepted June 1, 2000

Although hexavalent chromium has been shown to induce apoptosis in cells cultivated in vitro, there appear to be no studies focusing on the dynamics of this process. To find out about dynamic patterns of hexavalent chromium-induced apoptosis, we treated Hep2 cells with 150 μg/ml potassium chromate and recorded their behavior as well as appearance of some crucial organelles using different morphological and biochemical methods. We found that Hep2 cells showed the earliest observable changes at 6 hours after the treatment (blebbing, chromatin shrinkage), with the entire apoptotic process lasting up to 24 hours. While all the observed cell features clearly prove apoptosis induced by hexavalent chromium, a typical apoptotic hallmark, DNA ladder, seems not to occur in this type of cells. On the other hand, in HL-60 cells, used as a control, this ladder was observable.


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