EUS Guided FNA Cell Block Cytology and Intraoperative Squash Cytology in the Diagnostic Approach of Unfamiliar Malignant Neoplastic Disorders

Kalogeraki, Alexandra; Tamiolakis, Dimitrios; Moustou, Eleni; Kalaitzakis, Evangelos

doi:10.14712/18059694.2025.22

Acta Medica > Archive > 2025, Vol. 68 > Issue 2 > EUS Guided FNA Cell Block Cytology and…

Acta Med. 2025, 68: 67-72

https://doi.org/10.14712/18059694.2025.22

EUS Guided FNA Cell Block Cytology and Intraoperative Squash Cytology in the Diagnostic Approach of Unfamiliar Malignant Neoplastic Disorders

Alexandra Kalogeraki^a, Dimitrios Tamiolakis^b, Eleni Moustou^a, Evangelos Kalaitzakis^b

^aDepartments of Pathology-Cytopathology, Medical School, University of Crete, University Hospital, Heraklion, Crete, Greece
^bGastroenterology, Medical School, University of Crete, University Hospital, Heraklion, Crete, Greece

Received March 31, 2025
Accepted August 12, 2025

Endoscopic ultrasound guided fine needle aspiration cytology (EUS-FNAC) with the employment of cell block preparations and intraoperative squash smear cytology upgrade the interpretation accuracy and typing of common malignant lesions. Yet, their capacity in the diagnostic workup of less familiar neoplastic entities is not clearly determined and this analysis was designed towards this direction. We describe four cases of patients with uncommon malignancies and evaluate EUS-FNA cell block cytology and intraoperative squash smear cytology as a necessary (important) step in rendering the diagnosis. All cases enhance the diagnostic role of cytology in a wide variety of neoplastic disorders including lymphoproliferative conditions and rare carcinomas.