Acta Med. 2019, 62: 170-173

Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPLC Technique: a Preliminary Report

Kirill V. Ermakova, Alexander A. Bukhvostova, Alexander S. Vedenkinb, Sergey V. Stovbunb, Anton S. Dvornikova, Dmitry A. Kuznetsova,b

aN.I. Pirogov Russian National Research Medical University, Russian Federal Ministry of Health, Ostrovityanov St., 1, Moscow 117997, Russia
bN.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Kosygin St., 4, Moscow 119991, Russia

Received July 26, 2019
Accepted October 7, 2019

A significant population of ultrashort (50–150n) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng mL−1), but not in plasma of healthy donors. An original high resolution HPLC technique has been proposed to reveal and characterize this peculiarity. To solve this task, a novel molecular size exclusion – anion exchange analytical technique was developed. Its applicability to diagnostics and oncogenesis research is quizzed here.


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