Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPLC Technique: a Preliminary Report

Ermakov, Kirill V.; Bukhvostov, Alexander A.; Vedenkin, Alexander S.; Stovbun, Sergey V.; Dvornikov, Anton S.; Kuznetsov, Dmitry A.

doi:10.14712/18059694.2020.8

Acta Medica > Archive > 2019, Vol. 62 > Issue 4 > Ultrashort ssDNA in Retinoblastoma…

Acta Med. 2019, 62: 170-173

https://doi.org/10.14712/18059694.2020.8

Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPLC Technique: a Preliminary Report

Kirill V. Ermakov^a, Alexander A. Bukhvostov^a, Alexander S. Vedenkin^b, Sergey V. Stovbun^b, Anton S. Dvornikov^a, Dmitry A. Kuznetsov^a,b

^aN.I. Pirogov Russian National Research Medical University, Russian Federal Ministry of Health, Ostrovityanov St., 1, Moscow 117997, Russia
^bN.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Kosygin St., 4, Moscow 119991, Russia

Received July 26, 2019
Accepted October 7, 2019

A significant population of ultrashort (50–150n) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng mL⁻¹), but not in plasma of healthy donors. An original high resolution HPLC technique has been proposed to reveal and characterize this peculiarity. To solve this task, a novel molecular size exclusion – anion exchange analytical technique was developed. Its applicability to diagnostics and oncogenesis research is quizzed here.