Modulation of Rat Liver Regeneration after Partial Hepatectomy by Dietary Cholesterol

Živný, Pavel; Živná, Helena; Palička, Vladimír; Žaloudková, Lenka; Mocková, Petra; Cermanová, Jolana; Mičuda, Stanislav

doi:10.14712/18059694.2018.19

Acta Medica > Archive > 2018, Vol. 61 > Issue 1 > Modulation of Rat Liver Regeneration…

Acta Med. 2018, 61: 22-28

https://doi.org/10.14712/18059694.2018.19

Modulation of Rat Liver Regeneration after Partial Hepatectomy by Dietary Cholesterol

Pavel Živný^a, Helena Živná^b

, Vladimír Palička^a

, Lenka Žaloudková^a

, Petra Mocková^a, Jolana Cermanová^c

, Stanislav Mičuda^c

^aInstitute of Clinical Biochemistry and Diagnostics, University Hospital, Charles University, Hradec Králové, Czech Republic
^bRadioisotope Laboratories and Vivarium, Medical Faculty, Charles University, Hradec Králové, Czech Republic
^cDepartment of Pharmacology, Medical Faculty, Charles University, Hradec Králové, Czech Republic

Received January 10, 2018
Accepted May 13, 2018

Introduction: The aim of study was to evaluate impact of long-term dietary cholesterol overload on the cholesterol homeostasis and liver regeneration. Material and Methods: Serum lipid parameters, ¹⁴C-cholesterol incorporation, liver DNA synthesis and protein expression was determined in partially hepatectomized (PH) rats fed with a standard (SLD) or hypercholesterolemic (CHOL) diet. Results: 29-day intake of CHOL diet before PH produced increase in serum total cholesterol, LDL lipoprotein, and triglyceride concentration. PH provoked decrease in serum total cholesterol and triglyceride concentration in both groups. PH was associated with increase in serum ALT activity more pronounced in CHOL animals. Hepatic DNA synthesis was increased after PH in both groups, but lower in CHOL. Hypercholesterolemic diet reduced the absorption of radiolabelled cholesterol in intestine and then activity in blood and liver. The ¹⁴C-cholesterol hepatic activities tend to increase after PH in both groups. CHOL diet produced up-regulation of Acyl-CoA:cholesterol acyltransferase-2 protein expression. PH was associated with increase of LDL receptor and Acyl-CoA:cholesterol acyltransferase-2 protein expression in both dietary groups. Discussion: Liver regeneration after PH is negatively influenced by CHOL diet. The increased uptake of cholesterol in the liver after PH associated with up-regulation of LDL receptor protein expression suggests preferential use of extrahepatic cholesterol by the liver.