Acta Med. 2016, 59: 91-96

Effect of Riboflavin/UVA Collagen Cross-linking on Central Cornea, Limbus and Intraocular Pressure. Experimental Study in Rabbit Eyes

Vassilios Kozobolisa, Maria Gkikaa, Haris Sideroudia, Efthymia Tsaraglia, Stylliani Lydatakib,c, Irini Naoumidic, Alexandra Giatromanolakid, Dimitrios Mikropoulose, Miguel Teusf, Georgios Labirisa

aEye Institute of Thrace, Democritus University of Thrace, Alexandroupolis, Greece
bInstitut Carnot de Bourgogne, University of Burgundy, Bourgogne, France
cInstitute of Vision and Optics, University of Crete, Heraklion, Greece
dDepartment of Pathology, Democritus University of Thrace, Alexandroupolis, Greece
e1st University Department of Ophthalmology, Aristotle University of Thessaloniki, Thessaloniki, Greece
fUniversidad de Alcalá, Madrid, Spain

Received June 8, 2016
Accepted August 23, 2016

The Purpose of present study was to investigate the effect of riboflavin/ultraviolet-A-induced collagen cross-linking (CXL) on central cornea, limbus and intraocular pressure (IOP). This was an animal experimental study. The right corneas of 10 rabbits were ultraviolet-A irradiated (3 mW/cm2 for 30 minutes) after de-epithelialization and instillation of 0.1% riboflavin / 20% Dextran drops. Left corneas served as controls. Samples were examined histologically one month postoperatively. Before and after treatment, IOP measurements were recorded bilaterally. At central cornea of eyes underwent CXL keratocyte repopulation, normal arrangement of collagen fibres and a statistically significant change in fibres diameter were detected, compared to controls. At limbus area, there were not any significant histological differences after CXL. There was no statistically significant difference between pre- and postoperative IOP in all eyes.


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