Effect of Riboflavin/UVA Collagen Cross-linking on Central Cornea, Limbus and Intraocular Pressure. Experimental Study in Rabbit Eyes

Kozobolis, Vassilios; Gkika, Maria; Sideroudi, Haris; Tsaragli, Efthymia; Lydataki, Stylliani; Naoumidi, Irini; Giatromanolaki, Alexandra; Mikropoulos, Dimitrios; Teus, Miguel; Labiris, Georgios

doi:10.14712/18059694.2016.96

Acta Medica > Archive > 2016, Vol. 59 > Issue 3 > Effect of Riboflavin/UVA Collagen…

Acta Med. 2016, 59: 91-96

https://doi.org/10.14712/18059694.2016.96

Effect of Riboflavin/UVA Collagen Cross-linking on Central Cornea, Limbus and Intraocular Pressure. Experimental Study in Rabbit Eyes

Vassilios Kozobolis^a, Maria Gkika^a, Haris Sideroudi^a, Efthymia Tsaragli^a, Stylliani Lydataki^b,c, Irini Naoumidi^c, Alexandra Giatromanolaki^d, Dimitrios Mikropoulos^e, Miguel Teus^f, Georgios Labiris^a

^aEye Institute of Thrace, Democritus University of Thrace, Alexandroupolis, Greece
^bInstitut Carnot de Bourgogne, University of Burgundy, Bourgogne, France
^cInstitute of Vision and Optics, University of Crete, Heraklion, Greece
^dDepartment of Pathology, Democritus University of Thrace, Alexandroupolis, Greece
^e1st University Department of Ophthalmology, Aristotle University of Thessaloniki, Thessaloniki, Greece
^fUniversidad de Alcalá, Madrid, Spain

Received June 8, 2016
Accepted August 23, 2016

The Purpose of present study was to investigate the effect of riboflavin/ultraviolet-A-induced collagen cross-linking (CXL) on central cornea, limbus and intraocular pressure (IOP). This was an animal experimental study. The right corneas of 10 rabbits were ultraviolet-A irradiated (3 mW/cm² for 30 minutes) after de-epithelialization and instillation of 0.1% riboflavin / 20% Dextran drops. Left corneas served as controls. Samples were examined histologically one month postoperatively. Before and after treatment, IOP measurements were recorded bilaterally. At central cornea of eyes underwent CXL keratocyte repopulation, normal arrangement of collagen fibres and a statistically significant change in fibres diameter were detected, compared to controls. At limbus area, there were not any significant histological differences after CXL. There was no statistically significant difference between pre- and postoperative IOP in all eyes.