Acta Med. 2015, 58: 21-24

Melatonin Regulates Oxidative Stress Initiated by Freund’s Complete Adjuvant

Miroslav Pohankaa, Branislav Ruttkay-Nedeckýb,c, Josef Fuseka, Vojtěch Adamb,c, René Kizekb,c

aFaculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic
bCentral European Institute of Technology, Brno University of Technology, Brno, Czech Republic
cDepartment of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic

Received February 19, 2015
Accepted March 31, 2015

Melatonin is a hormone with strong antioxidant properties. In this experiment, Freund’s complete adjuvant was used as a stressogenic substance given to laboratory outbred mice, whereas melatonin was investigated as a protectant against the stressogenic effect. Levels of low molecular weight antioxidants, thiobarbituric acid reactive substances, and tumor necrosis factor α and activity of glutathione reductase were determined in blood from the animals. Surprisingly, melatonin was not involved in direct regulation of antioxidants, thiobarbituric acid reactive substances and tumor necrosis factor α. On the other hand, melatonin regulated glutathione reductase activity. We can conclude on regulation of metabolism caused by melatonin in the model. The effect was more important than the expected regulation of immunity and basal oxidative homeostasis.


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