The TT Genotype of the MTHFR 677C>T Polymorphism Increases Susceptibility to Premature Coronary Artery Disease in Interaction with Some of the Traditional Risk Factors

Sarecka-Hujar, Beata; Zak, Iwona; Krauze, Jolanta

doi:10.14712/18059694.2015.42

Acta Medica > Archive > 2012, Vol. 55 > Issue 4 > The TT Genotype of the MTHFR 677C>T…

Acta Med. 2012, 55: 172-179

https://doi.org/10.14712/18059694.2015.42

The TT Genotype of the MTHFR 677C>T Polymorphism Increases Susceptibility to Premature Coronary Artery Disease in Interaction with Some of the Traditional Risk Factors

Beata Sarecka-Hujar^a, Iwona Zak^b, Jolanta Krauze^c

^aDepartment of Applied Pharmacy, Medical University of Silesia, Sosnowiec, Poland
^bDepartment of Biochemistry and Medical Genetics, Medical University of Silesia, Katowice, Poland
^cThe First Department of Cardiac Surgery, Medical University of Silesia, Katowice, Poland

Received March 20, 2012
Accepted October 10, 2012

Background: The presence of several risk factors (genetic and non-genetic) has greater impact on the risk of premature coronary artery disease (CAD) than single risk factor. Objective: The aim of the study was to establish possible relations between genotypes and alleles of 677C>T polymorphism of MTHFR gene and some traditional risk factors e.g. elevated levels of lipid parameters and smoking in development of premature CAD. Methods: The groups comprised 152 patients with angiographically documented premature CAD (aged 42.9 ± 5.5) and 121 age-matched blood donors (aged 42.3 ± 6.5) were studied. The MTHFR 677C>T polymorphism was genotyped with Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. Results: Patients with TT genotype who simultaneously smoked had increased risk of premature CAD compared to non-smoking cases with CC genotype (OR = 24.62). We also found that individuals with TT genotype and elevated LDL-cholesterol (LDL-chol.) level had significantly higher risk of CAD (OR = 9.92) than individuals with normal LDL-chol. level and CC genotype. Conclusions: The present study shows that simultaneous presence of MTHFR TT genotype and smoking or elevated levels of LDL-chol. influences the risk of premature CAD. This findings give interesting contribution to gene-environment interaction problem that may have clinical implications in the future.