Human Papillomavirus Genotype Spectrum in Studied Group of Montenegrin Women

Vujošević, Danijela; Vuksanović, Vineta; Poljak, Mario; Jokmanović, Nebojša

doi:10.14712/18059694.2015.50

Acta Medica > Archive > 2012, Vol. 55 > Issue 3 > Human Papillomavirus Genotype Spectrum…

Acta Med. 2012, 55: 130-132

https://doi.org/10.14712/18059694.2015.50

Human Papillomavirus Genotype Spectrum in Studied Group of Montenegrin Women

Danijela Vujošević^a, Vineta Vuksanović^a,b, Mario Poljak^c, Nebojša Jokmanović^d

^aCenter for Medical Microbiology, Institute of Public Health, Podgorica, Montenegro
^bMedical Faculty, University of Montenegro, Podgorica, Montenegro
^cInstitute of Microbiology and Immunology, Faculty of Medicine, Ljubljana, Slovenia
^dClinic for Gynecology and Obstetrics, Clinical Center of Montenegro, Podgorica, Montenegro

Received February 7, 2011
Accepted September 5, 2012

The risk of high-grade lesions and carcinoma is known to correlate with specific high-risk HPV genotypes. The distribution of HPV types varies between countries and little is known about HPV distribution in our country. Therefore, the purpose of this study was to determine the range and frequency of HPV genotypes in studied group of women in Montenegro. HPV genotypes were determined using the method of enzyme restriction of PCR products amplified with group-specific primers MY09/MY11 and restricted with seven different restriction endonucleases. Out of the total number of women HPV infection was found in 1/5 of participants (20%). Genotyping performed in HPV DNA positive women shows that the HPV genotype 16 is dominant and present in more than 1/3 of the participants (36.8%). The second most frequent HPV infection is with HPV genotype 58 and it is found in 10.5% of participants. HPV 31 and HPV 6 infections are present in 7.9% of women, while infections with other genotypes were demonstrated individually by 2.6%. Multiple HPV infection was demonstrated in 18.4% and they are dominant in younger women (aged 25 to 30 years).