Acta Med. 2011, 54: 111-116
Analysis of D1853N ATM Polymorphism in Radiosensitive Patients with Cervical Carcinoma
Clinical oncologists have been focusing their efforts on attempting to define risk groups of patients with unusual biological reactions to the recommended therapy regimens using molecular biology techniques. The aims of our study were: (i) to find a design and validate a method for fast and reliable analysis of the D1853N (5557G>A) genetic polymorphism in the ATM (ataxia-telangiectasia mutated) gene; (ii) to use side-directed mutagenesis to generate ATM 5557A-positive DNA (reference ATM5557A DNA); and (iii) to analyze a group of patients suffering from cervical carcinoma with adverse responses to radiotherapy. The 5557A variant was found in three of twenty women (15%). Our data show that the prevalence of the 5557A allelic variant in cervical cancer subjects with adverse responses after irradiation probably does not differ from the prevalence common in Caucasians. A larger population study should confirm these preliminary results.
Keywords
ATM, Mutation, Genotyping, Oncology, Diagnostics, Treatment, DNA, PCR, RFLP.
Funding
The study was supported by research projects NT11334- 4/2010 and MZO 00179906 from the Ministry of Health, Czech Republic.
References
Copyright
Published by the Karolinum Press. For permission to use please write to actamedica@lfhk.cuni.cz.
