Acta Med. 2010, 53: 93-99

https://doi.org/10.14712/18059694.2016.66

Stem Cells from Human Exfoliated Deciduous Teeth – Isolation, Long Term Cultivation and Phenotypical Analysis

Jakub Sucháneka, Benjamín Víšekb, Tomáš Soukupb, Sally Kamal El-Din Mohamedc, Romana Ivančakováa, Jaroslav Mokrýb, Eman H. A. Aboul-Ezzc, A. Omrand

aCharles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Dentistry, Hradec Králové, Czech Republic
bCharles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Histology and Embryology, Hradec Králové, Czech Republic
cOro-Dental Genetic Department, National Research Center, Cairo, Egypt
dFaculty of Dentistry, Ein-Shams University, Cairo, Egypt

Received February 8, 2010
Accepted May 6, 2010

References

1. Chai Y, Slavkin HC. Prospects for tooth regeneration in the 21st century: A perspective. Microscopy research and technique. 2003; 60: 469–479. <https://doi.org/10.1002/jemt.10287>
2. Gotlieb E. L, Murray P. E, Namerow K. N, Kuttler S, Garcia-Godoy F. An ultrastructural investigation of tissue-engineered pulp constructs implanted within endodontically treated teeth. J Am Dent Assoc 2008; 139: 457–465. <https://doi.org/10.14219/jada.archive.2008.0189>
3. Gronthos S, Cherman N, Robey P, Shi S. Human dental pulp stem cells. Adult Stem Cells. Totowa, New Jersey: Humana Press, 2004: 37–51,101–149.
4. Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA 2000; 97:13625–13630. <https://doi.org/10.1073/pnas.240309797> <PubMed>
5. Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG, Shi S. SHED: Stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci USA 2003; 100:5807–5812. <https://doi.org/10.1073/pnas.0937635100> <PubMed>
6. Pereira, R.F., Halford, K.W., O’Hara, M.D., et al.: Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage and lung in irradiated mice. Proc Natl Acad Sci USA, 1995, č. 92, s. 4857–61. <https://doi.org/10.1073/pnas.92.11.4857> <PubMed>
7. Sanchez-Ramos, J. et al.: Adult bone marrow stromal cells differentiate into neural cells in vitro. Exp Neurol, 2000, č. 164, s. 247–256. <https://doi.org/10.1006/exnr.2000.7389>
8. Suchánek J, Soukup T, Ivančaková R, et al. Human dental pulp stem cell – isolation and long term cultivation. Acta Med (Hradec Kralove) 2007; 50: 195–201. <https://doi.org/10.14712/18059694.2017.82>
9. Suchánek J., Soukup T., Víšek B., et al. Dental pulp stem cells and their characterization. Biomed Pap Med Fac Univ Palacky Olomouc Czech Rep. 2009, 153(1):1–6.
10. Turksen K. Adult Stem Cells. Totowa, New Jersey: Humana Press, 2004:37–51, 101–149.
11. Yen, AHH; Sharpe, PT. Regeneration of teeth using stem cell-based tissue engineering. Expert opinion on biological therapy. 2006; 6: 9–16. <https://doi.org/10.1517/14712598.6.1.9>
12. Yu J, Deng Z, Shi J, Zhai H, Nie X, Zhuang H, Li Y, Jin Y. Differentiation of dental pulp cells into regular-shaped dentin pulp complex induced by tooth germ cell conditioned medium. Tissue Eng. 2006; 11:3097–3105. <https://doi.org/10.1089/ten.2006.12.3097>
front cover

ISSN 1211-4286 (Print) ISSN 1805-9694 (Online)

Open access journal

Archive