Acta Med. 2010, 53: 79-84

https://doi.org/10.14712/18059694.2016.64

Osteogenic Differentiation of Human Dental Pulp-derived Stem Cells under Various Ex-vivo Culture Conditions

Jana Karbanováa,b, Tomáš Soukupa, Jakub Suchánekc, Jaroslav Mokrýa

aCharles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Histology and Embryology, Hradec Králové, Czech Republic
bTissue Engineering Laboratories, BIOTEC, Technische Universität Dresden, Germany
cCharles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Dentistry, Hradec Králové, Czech Republic

Received March 5, 2010
Accepted March 19, 2010

References

1. Arthur A, Rychkov G, Shi S, Koblar SA, Gronthos S. Adult human dental pulp stem cells differentiate toward functionally active neurons under appropriate environmental cues. Stem Cells. 2008; 26(7):1787–95. <https://doi.org/10.1634/stemcells.2007-0979>
2. Chai Y, Jiang X, Ito Y, Bringas P, Jr., Han J, Rowitch DH, et al. Fate of the mammalian cranial neural crest during tooth and mandibular morphogenesis. Development. 2000; 127(8):1671–9.
3. Chen D, Zhao M, Mundy GR. Bone morphogenetic proteins. Growth Factors. 2004; 22(4):233–41. <https://doi.org/10.1080/08977190412331279890>
4. Cho HH, Park HT, Kim YJ, Bae YC, Suh KT, Jung JS. Induction of osteogenic differentiation of human mesenchymal stem cells by histone deacetylase inhibitors. J Cell Biochem. 2005; 96(3):533–42. <https://doi.org/10.1002/jcb.20544>
5. d’Aquino R, De Rosa A, Lanza V, Tirino V, Laino L, Graziano A, et al. Human mandible bone defect repair by the grafting of dental pulp stem/progenitor cells and collagen sponge biocomplexes. Eur Cell Mater. 2009; 18:75–83. <https://doi.org/10.22203/eCM.v018a07>
6. Gronthos S, Brahim J, Li W, Fisher LW, Cherman N, Boyde A, et al. Stem cell properties of human dental pulp stem cells. J Dent Res. 2002; 81(8):531–5. <https://doi.org/10.1177/154405910208100806>
7. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A. 2000; 97(25):13625–30. <https://doi.org/10.1073/pnas.240309797> <PubMed>
8. Hanada K, Dennis JE, Caplan AI. Stimulatory effects of basic fibroblast growth factor and bone morphogenetic protein-2 on osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells. J Bone Miner Res. 1997; 12(10): 1606–14. <https://doi.org/10.1359/jbmr.1997.12.10.1606>
9. Hsieh J, Nakashima K, Kuwabara T, Mejia E, Gage FH. Histone deacetylase inhibition- mediated neuronal differentiation of multipotent adult neural progenitor cells. Proc Natl Acad Sci U S A. 2004; 101(47):16659–64. <https://doi.org/10.1073/pnas.0407643101> <PubMed>
10. Ignotz RA, Massague J. Transforming growth factor-beta stimulates the expression of fibronectin and collagen and their incorporation into the extracellular matrix. J Biol Chem. 1986; 261(9):4337–45.
11. Kerkis I, Kerkis A, Dozortsev D, Stukart-Parsons GC, Gomes Massironi SM, Pereira LV, et al. Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers. Cells Tissues Organs. 2006; 184(3–4):105–16. <https://doi.org/10.1159/000099617>
12. Laino G, d’Aquino R, Graziano A, Lanza V, Carinci F, Naro F, et al. A new population of human adult dental pulp stem cells: a useful source of living autologous fibrous bone tissue (LAB). J Bone Miner Res. 2005; 20(8):1394–402. <https://doi.org/10.1359/JBMR.050325>
13. Laino G, Graziano A, d’Aquino R, Pirozzi G, Lanza V, Valiante S, et al. An approachable human adult stem cell source for hard-tissue engineering. J Cell Physiol. 2006; 206(3):693–701. <https://doi.org/10.1002/jcp.20526>
14. Lopez-Cazaux S, Bluteau G, Magne D, Lieubeau B, Guicheux J, Alliot-Licht B. Culture medium modulates the behaviour of human dental pulp-derived cells: technical note. Eur Cell Mater. 2006; 11:35–42. <https://doi.org/10.22203/eCM.v011a05>
15. Mackay AM, Beck SC, Murphy JM, Barry FP, Chichester CO, Pittenger MF. Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow. Tissue Eng. 1998; 4(4):415–28. <https://doi.org/10.1089/ten.1998.4.415>
16. Marie PJ. Fibroblast growth factor signaling controlling osteoblast differentiation. Gene. 2003; 316:23–32. <https://doi.org/10.1016/S0378-1119(03)00748-0>
17. Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG, et al. SHED: stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci U S A. 2003; 100(10): 5807–12. <https://doi.org/10.1073/pnas.0937635100> <PubMed>
18. Pri-Chen S, Pitaru S, Lokiec F, Savion N. Basic fibroblast growth factor enhances the growth and expression of the osteogenic phenotype of dexamethasone-treated human bone marrow-derived bone-like cells in culture. Bone. 1998; 23(2):111–7. <https://doi.org/10.1016/S8756-3282(98)00087-8>
19. Proudfoot D, Skepper JN, Shanahan CM, Weissberg PL. Calcification of human vascular cells in vitro is correlated with high levels of matrix Gla protein and low levels of osteopontin expression. Arterioscler Thromb Vasc Biol. 1998; 18(3): 379–88. <https://doi.org/10.1161/01.ATV.18.3.379>
20. Schroeder TM, Westendorf JJ. Histone deacetylase inhibitors promote osteoblast maturation. J Bone Miner Res. 2005; 20(12):2254–63. <https://doi.org/10.1359/JBMR.050813>
21. Stevens A, Zuliani T, Olejnik C, LeRoy H, Obriot H, Kerr-Conte J, et al. Human dental pulp stem cells differentiate into neural crest-derived melanocytes and have label-retaining and sphere-forming abilities. Stem Cells Dev. 2008; 17(6):1175–84. <https://doi.org/10.1089/scd.2008.0012>
22. Suchanek J, Soukup T, Ivancakova R, Karbanova J, Hubkova V, Pytlik R, et al. Human dental pulp stem cells—isolation and long term cultivation. Acta Medica (Hradec Kralove). 2007; 50(3):195–201. <https://doi.org/10.14712/18059694.2017.82>
23. Yalvac ME, Ramazanoglu M, Rizvanov AA, Sahin F, Bayrak OF, Salli U, et al. Isolation and characterization of stem cells derived from human third molar tooth germs of young adults: implications in neo-vascularization, osteo-, adipo- and neurogenesis. Pharmacogenomics J. 2010; 10(2):105–13. <https://doi.org/10.1038/tpj.2009.40>
front cover

ISSN 1211-4286 (Print) ISSN 1805-9694 (Online)

Open access journal

Archive