Acta Med. 2009, 52: 101-106
The Effect of Interferon-γ and Lipopolysaccharide on the Growth of Francisella tularensis LVS in Murine Macrophage-like Cell Line J774
Background: Francisella tularensis, a causative agent of human tularemia, displaying the ability to proliferate inside the human cells. Aims: To evaluate the growth potential of F. tularensis LVS strain in macrophage-like cell line J774 modulated by recombinant interferon γ and E. coli derived lipopolysaccharide. Results: Stimulation of J774 cells either by interferon-γ or lipopolysaccharide alone, or especially in combination before infection F. tularensis, revealed protective effects. Higher concentrations of stimulating agents were needed to inhibit ongoing F. tularensis infection. Conclusions: Stimulation of J774 cell line by combination of interferon-γ with lipopolysaccharide inhibits the intracellular growth of F. tularensis.
Keywords
Francisella tularensis, Macrophage, Interferon-γ, Lipopolysaccharide.
Funding
The presented study was fully supported by Ministry of Education, Youth and Sport, Czech Republic, grant No. LN00A033.
References
Copyright
Published by the Karolinum Press. For permission to use please write to actamedica@lfhk.cuni.cz.