Quantitative Analysis of Expression Level of BCL2 and BAX Genes in Hep-2 and HL-60 Cells after Treatment with Etoposide

Královcová, Dita; Pejchalová, Marcela; Rudolf, Emil; Červinka, Miroslav

doi:10.14712/18059694.2017.23

Acta Medica > Archive > 2008, Vol. 51 > Issue 3 > Quantitative Analysis of Expression…

Acta Med. 2008, 51: 191-195

https://doi.org/10.14712/18059694.2017.23

Quantitative Analysis of Expression Level of BCL2 and BAX Genes in Hep-2 and HL-60 Cells after Treatment with Etoposide

Dita Královcová^a, Marcela Pejchalová^a, Emil Rudolf^b, Miroslav Červinka^b

^aUniversity of Pardubice, Faculty of Chemical-Technology, Department of Biological and Biochemical Sciences, Pardubice, Czech Republic
^bCharles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biology and Genetics, Hradec Králové, Czech Republic

Received February 1, 2008
Accepted August 1, 2008

Apoptotic cell death is a highly regulated process, which plays a crucial role in many biological events. The aim of the present research was to investigate the expression of the apoptosis related genes BCL2 and BAX in Hep-2 and HL- 60 cells. Apoptosis was induced in these cell lines during treatment with etoposide. The expression levels of BCL2 and BAX genes were measured after 6 h and 12 h of treatment by quantitative real-time RT-PCR. In Hep-2 cells the expression level of BCL2 significantly increased both 6 h and 12 h of treatment, whereas expression level of BAX didn’t change. In HL-60 cells the expression level of BCL2 decreased after 6 h of treatment and expression of BAX increased both 6 h and 12 h of treatment with etoposide. Those findings show distinct reactions of Hep-2 and HL-60 cells to etoposide treatment and different upregulation or downregulation of apoptosis-related genes BCL2 and BAX.