The Expression of CD38 ADP-ribosyl Cyclase Ectoenzyme in Immune Cells of Cardiac Surgical Patients

Koláčková, Martina; Trojáčková Kudlová, Manuela; Lonský, Vladimír; Manďák, Jiří; Kuneš, Pavel; Jankovičová, Karolína; Vlášková, Dana; Andrýs, Ctirad; Krejsek, Jan

doi:10.14712/18059694.2017.5

Acta Medica > Archive > 2008, Vol. 51 > Issue 1 > The Expression of CD38 ADP-ribosyl…

Acta Med. 2008, 51: 31-35

https://doi.org/10.14712/18059694.2017.5

The Expression of CD38 ADP-ribosyl Cyclase Ectoenzyme in Immune Cells of Cardiac Surgical Patients

Martina Koláčková^a, Manuela Trojáčková Kudlová^a, Vladimír Lonský^b, Jiří Manďák^b, Pavel Kuneš^a,b, Karolína Jankovičová^a, Dana Vlášková^b, Ctirad Andrýs^a, Jan Krejsek^a

^aCharles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Clinical Immunology and Allergology, Hradec Králové, Czech Republic
^bCharles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Cardiac Surgery, Hradec Králové, Czech Republic

Received February 1, 2008
Accepted March 1, 2008

Background: This study was aimed at following the changes in the expression of CD38 ADP-ribosyl cyclase ectoenzyme on peripheral blood immune cells of patients undergoing cardiac surgical operations. Patients and Methods: The expression of CD38 on lymphoid and myeloid cells was determined by immunofluorescence and flow cytometry in forty cardiac surgical patients assigned to surgery either using (“on-pump”, n=20) or without the use (“off-pump”, n=20) of cardiopulmonary bypass. Results: There was a very rapid upregulation of CD38 expression in “on-pump” patients, becoming significant at declamping of aorta (p<0.01) for myeloid cells and at the weaning from CPB (p<0.001) for lymphocytes. The increased expression of CD38 on lymphocytes in “off-pump” patients was prolonged for the entire observation period. However, significant differences in the expression of CD38 between “on-pump” and “off-pump” patients were not found either in lymphoid or myeloid cells. Conclusion: CD38 expression in immune cells of cardiac surgical patients is upregulated early during surgery, providing additional activation stimuli to the cell substrate of the inflammatory response induced by cardiac surgery.

Keywords

Cardiac surgery, Immune cells, Activation, CD38, Expression.

Funding

This work was supported by Ministry of Education, Czech Republic, project No. MSM0021620812 and Internal Grant Agency of Ministry of Health, Czech Republic, No. NR/9090–4.