Acta Med. 2007, 50: 239-241

Amperometric Biosensor for Pesticide Methamidophos Assay

Miroslav Pohankaa, Kamil Kučab,c, Daniel Junb,c

aCenter of Biological Defense, Těchonín, Central Military Institute of Health, Czech Republic
bUniversity of Defence, Faculty of Military Health Sciences Hradec Králové, Center of Advanced Studies, Hradec Králové, Czech Republic
cUniversity of Defence, Faculty of Military Health Sciences Hradec Králové, Department of Toxicology, Hradec Králové, Czech Republic

Received September 1, 2007
Accepted October 1, 2007


1. Bajgar J. Organophosphates/nerve agent poisoning: mechanism of action, diagnosis, prophylaxis and treatment. Adv Clin Chem 2004; 38:151–216. <>
2. Bajgar J, Fusek J, Kuča K, Bartošová L, Jun D. Treatment of organophosphate intoxication using cholinesterase reactivators: facts and fiction. Mini Rev Med Chem 2007; 7:461–6. <>
3. Ciucu A, Ciucu C. Organic phase amperometric biosensor for detection of pesticides. 2002; 7:667–6.
4. Gulla KC, Gouda MD, Thakur MS, Karanth NG. Reactivation of immobilized acetylcholinesterae in an amperometric biosensor for organophosphorus pesticide. Biochim Biophys Acta 2002; 1597:133–9. <>
5. Hussain MA. Anticholines-terasse properties of methamidophos and acephate in insects and mammals. Bull Environ Contam Toxicol 1987; 38:131–8. <>
6. Inoue S, Saito T, Mase H et al. Rapid simultaneous determination for organophosphorus pesticides in human serum by LC-MS. J Pharm Biomed Anal 2007; 44:258:264. <>
7. Kassa J. Review of oximes in the antidotal treatment of poisoning by organophosphorus nerve agents. J Toxicol Clin Toxicol 2002; 6:803–16. <>
8. Kuča K, Jun D, Musílek K. Structural requirements of acetylcholinesterase reactivators. Mini Rev Med Chem 2006; 6:269–77. <>
9. Kuča K, Jun D, Bajgar J. Currently used cholinesterase reactivators against nerve agent intoxication: comparison of their effectivity in vitro. Drug Chem Toxicol 2007; 30:31–40. <>
10. Patočka J, Kuča K, Jun D. Acetylcholinesterase and butyrylcholinesterse – important enzymes of human body. Acta Medica 2004; 47:215–28.
11. Pohanka M, Jun D, Kuča K. Mycotoxin assays using biosensor technology: A review. Drug Chem Toxicol 2007; 30:253–61. <>
12. Pohanka M, Jun D, Kuča K. Amperometric biosensor for evaluation of competitive cholinesterase inhibition by the reactivator HI-6. Anal Lett In press.
13. Pohanka M, Skládal P, Kroča M. Biosensors for biological warfare agent detection. Def Sci J 2007; 57:185–93. <>
14. Pohanka M, Zbořil P. Amperometric biosensor for D-lactate assay. Food Technol Biotechnol In press.
15. Pozo OJ, Barreda M, Sancho JV et al. Multiresidue pesticide analysis of fruits by ultra-performance liquid chromaography tandem mass spectrometry. Anal Bioanal Chem In press.
16. Senanayake N, Johnson MK. Acute polyneuropathy after poisoning by a new organophosphate isecticide. N Engl J Med 1982; 306:155–7. <>
17. Senanayake N, Karalliedde L. Neurotoxic effects of organophospharus insecticides. N Engl J Med 1987; 316:761–3. <>
18. Skládal P. Biosensor based on cholinesterase for detection of pesticides. Food Technol Biotechnol 1996; 34:43–9.
19. Skládal P, Krejčí J. Performance of the amperometric biosensor with immobilized butyrylcholinesterase in organic solvents. Collect Czech Chem Commun 1996; 61:985–91. <>
20. Skládal P, Nunes GS, Yamanaka H, Ribeiro ML. Detection of carbamate pesticides in vegetable samples using cholinesterase-based biosensors. Electroanalysis 1997; 9:1083–7. <>
21. Sotiropoulou S, Chaniotakis NA. Lowereing the detection of the acetylcholinesterase biosensor using a nanoporous carbon matrix. Anal Chim Acta 2005; 530:199–204. <>
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