Acta Med. 2007, 50: 239-241

Amperometric Biosensor for Pesticide Methamidophos Assay

Miroslav Pohankaa, Kamil Kučab,c, Daniel Junb,c

aCenter of Biological Defense, Těchonín, Central Military Institute of Health, Czech Republic
bUniversity of Defence, Faculty of Military Health Sciences Hradec Králové, Center of Advanced Studies, Hradec Králové, Czech Republic
cUniversity of Defence, Faculty of Military Health Sciences Hradec Králové, Department of Toxicology, Hradec Králové, Czech Republic

Received September 1, 2007
Accepted October 1, 2007

Amperometric biosensor based on enzyme acetylcholinesterase (AChE; EC was tested for pesticide methamidophos assay. Biosensor consists from four screen printed platinum electrodes on ceramic strip. AChE was physically adsorbed onto the electrode surface. The measuring principle was based on the inhibition of AChE activity in the presence of methamidophos. The proposed method limit of detection was 2.45 nM, responding to 3.46 pg of methamidophos detected absolutely when we consider the sample volume.


The support of the Ministry of Industry and Trade of the Czech Republic for the Project No.2A-1TP1/007 is gratefully acknowledged.


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