Acta Med. 2007, 50: 195-201

https://doi.org/10.14712/18059694.2017.82

Human Dental Pulp Stem Cells – Isolation and Long Term Cultivation

Jakub Sucháneka, Tomáš Soukupb, Romana Ivančakováa, Jana Karbanováb, Věra Hubkováa, Robert Pytlíkc, Lenka Kučerovád

aCharles University in Prague, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Department of Dentistry, Hradec Králové, Czech Republic
bCharles University in Prague, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Department of Histology and Embryology, Hradec Králové, Czech Republic
cCharles University in Prague, 1st Medical Faculty in Prague, Teaching Hospital, 1st Department of Medicine, Prague, Czech Republic
dCharles University in Prague, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Department of Clinical Genetics, Hradec Králové, Czech Republic

Received March 1, 2007
Accepted August 1, 2007

Crossref Cited-by Linking

  • Badr Amany Elsaid, Amer Nouran Ahmad, Badawi Manal Farouk, Elbeltagi Mohamed Gamal: Effect of Boswellic Acid on Viability of Dental Pulp Stem Cells Compared to the Commonly Used Intracanal Medications: An In Vitro Study. The Journal of Contemporary Dental Practice 2024, 24, 957. <https://doi.org/10.5005/jp-journals-10024-3609>
  • Elbeltagy Mohamed G, Alazemi Mohammad J, Badawi Manal F, Badr Amany E: Examining the Effects of Asiaticoside on Dental Pulp Stem Cell Viability and Proliferation: A Promising Approach to Root Canal Treatment. The Journal of Contemporary Dental Practice 2024, 25, 118. <https://doi.org/10.5005/jp-journals-10024-3636>
  • Elbeltagy Mohamed G, Badawi Manal F, Badr Amany E, Alrashidi Mohammad A: The Effect of Glycyrrhizin on the Viability and Proliferation of Dental Pulp Stem Cells Compared to Intracanal Medicaments. The Journal of Contemporary Dental Practice 2024, 25, 267. <https://doi.org/10.5005/jp-journals-10024-3652>
  • Salkın Hasan, Basaran Kemal Erdem: Effects of non-steroidal anti-inflammatory drug (ibuprofen) in low and high dose on stemness and biological characteristics of human dental pulp-derived mesenchymal stem cells. Connective Tissue Research 2023, 64, 14. <https://doi.org/10.1080/03008207.2022.2083613>
  • Zhang Lili, Xia Dengsheng, Wang Chao, Gao Feifei, Hu Lei, Li Juan, Jin Luyuan: Pleiotrophin attenuates the senescence of dental pulp stem cells. Oral Diseases 2023, 29, 195. <https://doi.org/10.1111/odi.13929>
  • Salkin H., Acar M.B., Korkmaz S., Gunaydin Z., Gonen Z.B., Basaran K.E., Ozcan S.: Transforming growth factor β1-enriched secretome up-regulate osteogenic differentiation of dental pulp stem cells, and a potential therapeutic for gingival wound healing: A comparative proteomics study. Journal of Dentistry 2022, 124, 104224. <https://doi.org/10.1016/j.jdent.2022.104224>
  • Guo Meiling, Liu Fen, Wang Wenjuan, Liu Zhirong, Zhu Zhipeng, Liu Yiyu, Huang Zhen, Zia-Ul-Haq Muhammad: Naringin Promotes Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells via Wnt/β-Catenin. Evidence-Based Complementary and Alternative Medicine 2022, 2022, 1. <https://doi.org/10.1155/2022/4505471>
  • Khaseb Sanaz, Orooji Mahdi, Pour Majid Ghasemian, Safavi Seyed Mohammadreza, Eghbal Mohammad Jafar, Rezai Rad Maryam: Dental stem cell banking: Techniques and protocols. Cell Biology International 2021, 45, 1851. <https://doi.org/10.1002/cbin.11626>
  • Ebrahimi Maryam, Yaghoobi Mohammad Mehdi: Effects of aqueous and methanolic extracts of Astragalus Longistylus on growth and proliferation of human dental pulp stem cells. ADV TRADIT MED (ADTM) 2021, 21, 45. <https://doi.org/10.1007/s13596-020-00519-6>
  • Salkın Hasan, Gönen Zeynep Burçin, Özcan Servet, Bahar Dilek, Lekesizcan Ayça, Taheri Serpil, Kütük Nükhet, Alkan Alper: Effects of combination TGF-B1 transfection and platelet rich plasma (PRP) on three-dimension chondrogenic differentiation of rabbit dental pulp-derived mesenchymal stem cells. Connective Tissue Research 2021, 62, 226. <https://doi.org/10.1080/03008207.2019.1675649>
  • Pilbauerova Nela, Schmidt Jan, Soukup Tomas, Koberova Ivancakova Romana, Suchanek Jakub: The Effects of Cryogenic Storage on Human Dental Pulp Stem Cells. IJMS 2021, 22, 4432. <https://doi.org/10.3390/ijms22094432>
  • Gutiérrez-Quintero Juan G., Durán Riveros Juan Y., Martínez Valbuena Carlos A., Pedraza Alonso Sofía, Munévar JC, Viafara-García SM: Critical-sized mandibular defect reconstruction using human dental pulp stem cells in a xenograft model-clinical, radiological, and histological evaluation. Oral Maxillofac Surg 2020, 24, 485. <https://doi.org/10.1007/s10006-020-00862-7>
  • Ayoub Sara, Berbéri Antoine, Fayyad-kazan Mohammad: An update on human periapical cyst-mesenchymal stem cells and their potential applications in regenerative medicine. Mol Biol Rep 2020, 47, 2381. <https://doi.org/10.1007/s11033-020-05298-6>
  • Pokrywczynska Marta, Maj Małgorzata, Kloskowski Tomasz, Buhl Monika, Balcerczyk Daria, Jundziłł Arkadiusz, Szeliski Kamil, Rasmus Marta, Drewa Tomasz: Molecular Aspects of Adipose-Derived Stromal Cell Senescence in a Long-Term Culture: A Potential Role of Inflammatory Pathways. Cell Transplant 2020, 29, 096368972091734. <https://doi.org/10.1177/0963689720917341>
  • Awais Sadia, Balouch Samira Shabbir, Riaz Nabeela, Choudhery Mahmood S: Human Dental Pulp Stem Cells Exhibit Osteogenic Differentiation Potential. Open Life Sciences 2020, 15, 229. <https://doi.org/10.1515/biol-2020-0023>
  • Okajcekova Terezia, Strnadel Jan, Pokusa Michal, Zahumenska Romana, Janickova Maria, Halasova Erika, Skovierova Henrieta: A Comparative In Vitro Analysis of the Osteogenic Potential of Human Dental Pulp Stem Cells Using Various Differentiation Conditions. IJMS 2020, 21, 2280. <https://doi.org/10.3390/ijms21072280>
  • Nagaraja Shruthi, Mathew Sylvia, Abraham Anil, Ramesh Poornima, Chandanala Shashank: Evaluation of vascular endothelial growth factor - A release from platelet-rich fibrin, platelet-rich fibrin matrix, and dental pulp at different time intervals. J Conserv Dent 2020, 23, 359. <https://doi.org/10.4103/JCD.JCD_465_19>
  • Ferreira L. S., Diniz I. M. A., Maranduba C. M. S., Miyagi S. P. H., Rodrigues M. F. S. D., Moura-Netto C., Marques Márcia Martins: Short-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cells. Lasers Med Sci 2019, 34, 659. <https://doi.org/10.1007/s10103-018-2637-z>
  • Huang X., Liu F., Hou J., Chen K.: Inflammation‐induced overexpression of microRNA‐223‐3p regulates odontoblastic differentiation of human dental pulp stem cells by targeting SMAD3. Int Endodontic J 2019, 52, 491. <https://doi.org/10.1111/iej.13032>
  • Lin Tai-Chi, Lin Yi-Ying, Hsu Chih-Chen, Yang Yi-Ping, Yang Chang-Hao, Hwang De-Kuang, Wang Chien-Ying, Liu Yung-Yang, Lo Wen-Liang, Chiou Shih-Hwa, Peng Chi-Hsien, Chen Shih-Jen, Chang Yuh-Lih: Nanomedicine-based Curcumin Approach Improved ROS Damage in Best Dystrophy-specific Induced Pluripotent Stem Cells. Cell Transplant 2019, 28, 1345. <https://doi.org/10.1177/0963689719860130>
  • Lew Wei-Zhen, Huang Yu-Chih, Huang Kuen-Yu, Lin Che-Tong, Tsai Ming-Tzu, Huang Haw-Ming: Static magnetic fields enhance dental pulp stem cell proliferation by activating the p38 mitogen-activated protein kinase pathway as its putative mechanism. J Tissue Eng Regen Med 2018, 12, 19. <https://doi.org/10.1002/term.2333>
  • Xiao Jingwen, Cao Peipei, Wang Chenfei, Huang Dan, Lian Min, Song Yihua, Yin Weiwei, Zheng Ke, Gu Zhifeng, Gu Yongchun, Feng Guijuan, Feng Xingmei: The Forkhead Box C1, a Novel Negative Regulator of Osteogenesis, Plays a Crucial Role in Odontogenic Differentiation of Dental Pulp Stem Cells. Cellular Reprogramming 2018, 20, 312. <https://doi.org/10.1089/cell.2018.0011>
  • Bana Nikoo, Faghihi Faezeh, soleimani Mansooreh, Hayati Roodbari Nasim: Evaluating the differentiation potential of human chorion-derived mesenchymal stem cells into myoblast-like cells. MEDICAL SCIENCES 2018, 28, 195. <https://doi.org/10.29252/iau.28.3.195>
  • Ferrúa Camila P, do Amaral Cainá C, Giorgi Roberta, Garcia Tiago, Nedel Fernanda: How has Tooth Manipulation been Conducted for Dental Pulp Stem Cells Isolation? A Scoping Review. International Journal of Experimental Dental Science 2018, 7, 98. <https://doi.org/10.5005/jp-journals-10029-1182>
  • Rodas-Junco Beatriz A., Villicaña Claudia: Dental Pulp Stem Cells: Current Advances in Isolation, Expansion and Preservation. Tissue Eng Regen Med 2017, 14, 333. <https://doi.org/10.1007/s13770-017-0036-3>
  • Murakami Tomoya, Saitoh Issei, Sato Masahiro, Inada Emi, Soda Miki, Oda Masataka, Domon Hisanori, Iwase Yoko, Sawami Tadashi, Matsueda Kazunari, Terao Yutaka, Ohshima Hayato, Noguchi Hirofumi, Hayasaki Haruaki: Isolation and characterization of lymphoid enhancer factor-1-positive deciduous dental pulp stem-like cells after transfection with a piggyBac vector containing LEF1 promoter-driven selection markers. Archives of Oral Biology 2017, 81, 110. <https://doi.org/10.1016/j.archoralbio.2017.04.033>
  • Kruijt Spanjer Elmer C., Bittermann Gerhard K.P., van Hooijdonk Inge E.M., Rosenberg Antoine J.W.P., Gawlitta Debby: Taking the endochondral route to craniomaxillofacial bone regeneration: A logical approach?. Journal of Cranio-Maxillofacial Surgery 2017, 45, 1099. <https://doi.org/10.1016/j.jcms.2017.03.025>
  • He Xinyao, Jiang Wenkai, Luo Zhirong, Qu Tiejun, Wang Zhihua, Liu Ningning, Zhang Yaqing, Cooper Paul R., He Wenxi: IFN-γ regulates human dental pulp stem cells behavior via NF-κB and MAPK signaling. Sci Rep 2017, 7. <https://doi.org/10.1038/srep40681>
  • Bianco John, De Berdt Pauline, Deumens Ronald, des Rieux Anne: Taking a bite out of spinal cord injury: do dental stem cells have the teeth for it?. Cell. Mol. Life Sci. 2016, 73, 1413. <https://doi.org/10.1007/s00018-015-2126-5>
  • Nuti N., Corallo C., Chan B. M. F., Ferrari M., Gerami-Naini B.: Multipotent Differentiation of Human Dental Pulp Stem Cells: a Literature Review. Stem Cell Rev and Rep 2016, 12, 511. <https://doi.org/10.1007/s12015-016-9661-9>
  • Yusa Kazuyuki, Yamamoto Osamu, Iino Mitsuyoshi, Takano Hiroshi, Fukuda Masayuki, Qiao Zhiwei, Sugiyama Toshihiro: Eluted zinc ions stimulate osteoblast differentiation and mineralization in human dental pulp stem cells for bone tissue engineering. Archives of Oral Biology 2016, 71, 162. <https://doi.org/10.1016/j.archoralbio.2016.07.010>
  • Yusa Kazuyuki, Yamamoto Osamu, Takano Hiroshi, Fukuda Masayuki, Iino Mitsuyoshi: Zinc-modified titanium surface enhances osteoblast differentiation of dental pulp stem cells in vitro. Sci Rep 2016, 6. <https://doi.org/10.1038/srep29462>
  • Niehage Christian, Karbanová Jana, Steenblock Charlotte, Corbeil Denis, Hoflack Bernard, Papaccio Gianpaolo: Cell Surface Proteome of Dental Pulp Stem Cells Identified by Label-Free Mass Spectrometry. PLoS ONE 2016, 11, e0159824. <https://doi.org/10.1371/journal.pone.0159824>
  • Kleplová T., Browne K. Z., Soukup T., Suchánek J.: In Vitro Cultivation of Dental Pulp Stem Cells from Human Exfoliated Deciduous Teeth in Low-Xenogeneic-Serum Containing Media. ČSPZL 2016, 116, 3. <https://doi.org/10.51479/cspzl.2016.001>
  • Hata Masaki, Omi Maiko, Kobayashi Yasuko, Nakamura Nobuhisa, Tosaki Takahiro, Miyabe Megumi, Kojima Norinaga, Kubo Katsutoshi, Ozawa Shogo, Maeda Hatsuhiko, Tanaka Yoshinobu, Matsubara Tatsuaki, Naruse Keiko: Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells. Stem Cell Res Ther 2015, 6. <https://doi.org/10.1186/s13287-015-0156-4>
  • Sukarawan Waleerat, Nowwarote Nunthawan, Kerdpon Piyarat, Pavasant Prasit, Osathanon Thanaphum: Effect of basic fibroblast growth factor on pluripotent marker expression and colony forming unit capacity of stem cells isolated from human exfoliated deciduous teeth. Odontology 2014, 102, 160. <https://doi.org/10.1007/s10266-013-0124-3>
  • Zhu Lingxin, Yang Jingwen, Zhang Jie, Lei Dongqi, Xiao Lan, Cheng Xue, Lin Ying, Peng Bin: In vitro and in vivo evaluation of a nanoparticulate bioceramic paste for dental pulp repair. Acta Biomaterialia 2014, 10, 5156. <https://doi.org/10.1016/j.actbio.2014.08.014>
  • Kellner Manuela, Steindorff Marina M., Strempel Jürgen F., Winkel Andreas, Kühnel Mark P., Stiesch Meike: Differences of isolated dental stem cells dependent on donor age and consequences for autologous tooth replacement. Archives of Oral Biology 2014, 59, 559. <https://doi.org/10.1016/j.archoralbio.2014.02.014>
  • Chang Yun-Ching, Li Wan-Chun, Twu Nae-Fang, Li Hsin-Yang, Lo Wen-Liang, Chang Yuh-Lih, Lee Yi-Yen, Lin Chun-Fu, Shih Yang-Hsin, Chen Ming-Teh: Induction of dental pulp-derived induced pluripotent stem cells in the absence of c-Myc for differentiation into neuron-like cells. Journal of the Chinese Medical Association 2014, 77, 618. <https://doi.org/10.1016/j.jcma.2014.08.009>
  • Martin-Piedra Miguel Angel, Garzon Ingrid, Oliveira Ana Celeste, Alfonso-Rodriguez Camilo Andres, Carriel Victor, Scionti Giuseppe, Alaminos Miguel: Cell viability and proliferation capability of long-term human dental pulp stem cell cultures. Cytotherapy 2014, 16, 266. <https://doi.org/10.1016/j.jcyt.2013.10.016>
  • Ruparel Nikita B., de Almeida José Flávio Affonso, Henry Michael A., Diogenes Anibal: Characterization of a Stem Cell of Apical Papilla Cell Line: Effect of Passage on Cellular Phenotype. Journal of Endodontics 2013, 39, 357. <https://doi.org/10.1016/j.joen.2012.10.027>
  • Zhai Shafei, Wang Yafei, Jiang Wenkai, Jia Qian, Li Jie, Wang Wei, Wang Haijing, Ding Yonglin, Wang Ping, Liu Jun, Ni Longxing: Nemotic human dental pulp fibroblasts promote human dental pulp stem cells migration. Experimental Cell Research 2013, 319, 1544. <https://doi.org/10.1016/j.yexcr.2013.03.018>
  • Hata Masaki, Naruse Keiko, Ozawa Shogo, Kobayashi Yasuko, Nakamura Nobuhisa, Kojima Norinaga, Omi Maiko, Katanosaka Yuki, Nishikawa Toru, Naruse Keiji, Tanaka Yoshinobu, Matsubara Tatsuaki: Mechanical Stretch Increases the Proliferation While Inhibiting the Osteogenic Differentiation in Dental Pulp Stem Cells. Tissue Engineering Part A 2013, 19, 625. <https://doi.org/10.1089/ten.tea.2012.0099>
  • Nakashima Misako, Iohara Koichiro, Murakami Masashi: Dental pulp stem cells and regeneration. Endodontic Topics 2013, 28, 38. <https://doi.org/10.1111/etp.12027>
  • Suchánek Jakub, Suchánková Kleplová Tereza, Kapitán Martin, Soukup Tomáš: THE EFFECT OF FETAL CALF SERUM ON HUMAN DENTAL PULP STEM CELLS. Acta Med. (Hradec Kralove, Czech Repub.) 2013, 56, 142. <https://doi.org/10.14712/18059694.2014.9>
  • Cmielova Jana, Havelek Radim, Kohlerova Renata, Soukup Tomas, Bruckova Lenka, Suchanek Jakub, Vavrova Jirina, Mokry Jaroslav, Rezacova Martina: The effect of ATM kinase inhibition on the initial response of human dental pulp and periodontal ligament mesenchymal stem cells to ionizing radiation. International Journal of Radiation Biology 2013, 89, 501. <https://doi.org/10.3109/09553002.2013.775530>
  • Duailibi Monica Talarico, Kulikowski Leslie Domenici, Duailibi Silvio Eduardo, Lipay Monica Vannucci Nunes, Melaragno Maria Isabel, Ferreira Lydia Masako, Vacanti Joseph Phillip, Yelick Pamela Crotty: Cytogenetic instability of dental pulp stem cell lines. J Mol Hist 2012, 43, 89. <https://doi.org/10.1007/s10735-011-9373-z>
  • Wang Xi, Sha Xin-Jia, Li Guang-Hui, Yang Fu-Sheng, Ji Kun, Wen Ling-Ying, Liu Shi-Yu, Chen Lei, Ding Yin, Xuan Kun: Comparative characterization of stem cells from human exfoliated deciduous teeth and dental pulp stem cells. Archives of Oral Biology 2012, 57, 1231. <https://doi.org/10.1016/j.archoralbio.2012.02.014>
  • Chen Bo, Sun Hai-Hua, Wang Han-Guo, Kong Hui, Chen Fa-Ming, Yu Qing: The effects of human platelet lysate on dental pulp stem cells derived from impacted human third molars. Biomaterials 2012, 33, 5023. <https://doi.org/10.1016/j.biomaterials.2012.03.057>
  • Karbanová Jana, Soukup Tomáš, Suchánek Jakub, Pytlík Robert, Corbeil Denis, Mokrý Jaroslav: Characterization of Dental Pulp Stem Cells from Impacted Third Molars Cultured in Low Serum-Containing Medium. Cells Tissues Organs 2011, 193, 344. <https://doi.org/10.1159/000321160>
  • Muthna Darina, Soukup Tomas, Vavrova Jirina, Mokry Jaroslav, Cmielova Jana, Visek Benjamin, Jiroutova Alena, Havelek Radim, Suchanek Jakub, Filip Stanislav, English Denis, Rezacova Martina: Irradiation of Adult Human Dental Pulp Stem Cells Provokes Activation of p53, Cell Cycle Arrest, and Senescence but Not Apoptosis. Stem Cells and Development 2010, 19, 1855. <https://doi.org/10.1089/scd.2009.0449>
  • Mokry Jaroslav, Soukup Tomas, Micuda Stanislav, Karbanova Jana, Visek Benjamin, Brcakova Eva, Suchanek Jakub, Bouchal Jan, Vokurkova Doris, Ivancakova Romana: Telomere Attrition Occurs during Ex Vivo Expansion of Human Dental Pulp Stem Cells. Journal of Biomedicine and Biotechnology 2010, 2010, 1. <https://doi.org/10.1155/2010/673513>
  • Karbanová Jana, Soukup Tomáš, Suchánek Jakub, Mokrý Jaroslav: Osteogenic Differentiation of Human Dental Pulp-derived Stem Cells under Various Ex-vivo Culture Conditions. Acta Med. (Hradec Kralove, Czech Repub.) 2010, 53, 79. <https://doi.org/10.14712/18059694.2016.64>
  • Suchánek Jakub, Víšek Benjamín, Soukup Tomáš, El-Din Mohamed Sally Kamal, Ivančaková Romana, Mokrý Jaroslav, Aboul-Ezz Eman H. A., Omran A.: Stem Cells from Human Exfoliated Deciduous Teeth – Isolation, Long Term Cultivation and Phenotypical Analysis. Acta Med. (Hradec Kralove, Czech Repub.) 2010, 53, 93. <https://doi.org/10.14712/18059694.2016.66>
Crossref Cited-by Linking logo