Acta Med. 2007, 50: 195-201

https://doi.org/10.14712/18059694.2017.82

Human Dental Pulp Stem Cells – Isolation and Long Term Cultivation

Jakub Sucháneka, Tomáš Soukupb, Romana Ivančakováa, Jana Karbanováb, Věra Hubkováa, Robert Pytlíkc, Lenka Kučerovád

aCharles University in Prague, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Department of Dentistry, Hradec Králové, Czech Republic
bCharles University in Prague, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Department of Histology and Embryology, Hradec Králové, Czech Republic
cCharles University in Prague, 1st Medical Faculty in Prague, Teaching Hospital, 1st Department of Medicine, Prague, Czech Republic
dCharles University in Prague, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Department of Clinical Genetics, Hradec Králové, Czech Republic

Received March 1, 2007
Accepted August 1, 2007

References

1. Avery JK. Oral development and histology. 2nd ed. New York: Thieme Medical Publishers, Inc., 1994:71–79.
2. Gronthos S, Cherman N, Robey P, Shi S. Human dental pulp stem cells. Adult Stem Cells. Totowa, New Jersey: Humana Press, 2004:37–51,101–49.
3. Gronthos S, Mankani M, Brahim J et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA 2000; 97:13625–30. <https://doi.org/10.1073/pnas.240309797> <PubMed>
4. Hayflick L. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 1965: 2323–8.
5. Ivančaková R, Soukup T, Suchánek J, Karbanová J. Metodiky odběru zubní pulpy pro izolaci a kultivaci kmenových buněk. Čes. Stomat. 2006; 106(5): 131–5.
6. Jiang Y, Jahagirdar BN, Reinhardt L, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 2002; 418(4):41–49 + Supplementary Information www.nature.com/nature. <https://doi.org/10.1038/nature00870>
7. Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG, Shi S. SHED: Stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci USA 2003; 100:5807–12. <https://doi.org/10.1073/pnas.0937635100> <PubMed>
8. Minguel JJ, Erices A, Conget P. Mesenchymal stem cells. Exp Biol Med 2001; 226:507–20. <https://doi.org/10.1177/153537020122600603>
9. Morsczeck C, Reichrt TE, Völlner F, Gerlach T, Driemel O. The state of the art in human dental stem cell research. Mund Kiefer Gesichtschir 2007 Sep 6.
10. Reyes M, Lund T, Lenvik T, Aguiar D, Koodie L, Verfaillie CM. Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells. Blood 2001; 98(9):2615–25. <https://doi.org/10.1182/blood.V98.9.2615>
11. Soukup T, Mokrý J, Karbanová J, Pytlík R, Suchomel P, Kučerová L. Mesenchymal stem cells isolated from the human bone marrow: cultivation, phenotypic analysis and changes in proliferation kinetics. Acta Medica (Hradec Kralove). 2006; 49(1):27–33. <https://doi.org/10.14712/18059694.2017.106>
12. Turksen K. Adult Stem Cells. Totowa, New Jersey: Humana Press, 2004:37–51, 101–49.
front cover

ISSN 1211-4286 (Print) ISSN 1805-9694 (Online)

Open access journal

Archive