The Influence of Repeated Blood Withdrawals before Surgery on Clinical Outcome

Živná, Helena; Živný, Pavel; Palička, Vladimír; Šimáková, Eva; Řeháček, Vít

doi:10.14712/18059694.2017.69

Acta Medica > Archive > 2007, Vol. 50 > Issue 2 > The Influence of Repeated Blood…

Acta Med. 2007, 50: 129-133

https://doi.org/10.14712/18059694.2017.69

The Influence of Repeated Blood Withdrawals before Surgery on Clinical Outcome

Helena Živná^a, Pavel Živný^b, Vladimír Palička^b, Eva Šimáková^c, Vít Řeháček^d

^aCharles University in Prague, Faculty of Medicine and University Hospital in Hradec Králové, Radio-Isotope Laboratory and Vivarium, Hradec Králové, Czech Republic
^bCharles University in Prague, Faculty of Medicine and University Hospital in Hradec Králové, Institute for Clinical Biochemistry and Diagnostics, Hradec Králové, Czech Republic
^cCharles University in Prague, Faculty of Medicine and University Hospital in Hradec Králové, The Fingerland Department of Pathology, Hradec Králové, Czech Republic
^dCharles University in Prague, Faculty of Medicine and University Hospital in Hradec Králové, Transfusion Division, Hradec Králové, Czech Republic

Received February 1, 2007
Accepted May 1, 2007

The aim of this study was to find the influence of blood withdrawals and diet iron on elective surgery. Male Wistar rats (n=24) were divided: 1. group (SLD) ate standard laboratory diet (SLD), 2. group (FE) an iron enriched diet (FE) with one blood withdrawal after 9 weeks. 3. group (SLD-w) SLD and 4. group (FE-w) ate the FE diet; with 9 withdrawals once a week. The rats were sacrificed 18 hour after partial hepatectomy (PH) in the 10th week. Liver DNA synthesis (³H-thymidin – kBq/mg DNA) was performed. Serum hepcidin (pg/ml), iron concentration, respiratory burst of polymorfonucleares (RB, spontaneous; stimulated, %), count of blood cells were determined. FE-w had a higher (2.36±0.36) liver DNA synthesis after PH vs. SLD (1.21±0.49). Higher hemoglobin in erythrocytes (pg) was in FE-w and SLD-w vs. FE and SLD. PMN count in SLD-w, FE-w increased vs. SLD, FE. Hepcidin after PH decreased in SLD (78.0), FE (68.0), FE-w (97.0), but increased in SLD-w (217). Serum iron increased in SLD-w. RB after PH increased in FE-w (4.5; 47.6) vs. SLD (1.15; 29.1), FE (3.20;17.8), SLD-w (3.30;13.7). Conclusions: The iron diet with stimulation of haematopoesis by withdrawals improves an organism’s condition expressed as better response to elective surgery and better PMN functions.