Acta Med. 2004, 47: 327-329

https://doi.org/10.14712/18059694.2018.117

Assessment of Cellular Damage by Comet Assay After Photodynamic Therapy in vitro

Jaroslav Macečeka, Hana Kolářováa, Jitka Psotováb, Robert Bajgara, Martin Hufa, Pavla Nevřelováa, Marek Tomečkaa, Jiří Mosingerc

aPalacky University in Olomouc, Faculty of Medicine, Department of Medical Biophysics, Olomouc, Czech Republic
bPalacky University in Olomouc, Faculty of Medicine, Department of Medical Chemistry, Olomouc, Czech Republic
cCharles University in Prague, Faculty of Science, Department of Inorganic Chemistry, Prague, Czech Republic

References

1. Agarwal R, Korman NJ, Mohan RR et al. Apoptosis is an early event during phthalocyanine photodynamic therapy-induced ablation of chemically induced squamous papilomas in mouse skin. Photochem Photobiol 1996; 63(4):547–52. <https://doi.org/10.1111/j.1751-1097.1996.tb03082.x>
2. McNair FI, Marples B, West CML, Moore JV. A comet assay of DNA and repair in K562 cells after photodynamic therapy using haematoporphyrin derivate, methylene blue and meso-tetrahydroxyphenylchlorin. Br J Cancer 1997; 75(12): 1721–9. <https://doi.org/10.1038/bjc.1997.295> <PubMed>
3. Kessel D, Luo Y. Initiation of apoptosis versus necrosis by photodynamic therapy with chloroaluminium phtalocyanine. Photochem Photobiol 1997; 66(4): 479–83.
4. Mosinger J, Kliment V, Sejbal J et al. Host-guest complexes of anionic porphyrin sensitizers with cyclodextrins. J Porphyrins Phtalocyanines 2002; 6:513–25. <https://doi.org/10.1142/S1088424602000646>
5. Piperakis MP, Visvardis EE, Tassiou AM. Comet assay for nuclear DNA damage. Methods Enzymol 1999; 269(20):184–94. <https://doi.org/10.1016/S0076-6879(99)00125-1>
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