Acta Med. 2004, 47: 209-211

Visualization of Protein Aggregation in Nerve Cells After Ischemia/Reperfusion by Ubiquitin Immunohistochemistry and Impregnative Nauta Method

Eva Mechírová, Iveta Domoráková, Marianna Feriková

P.J. Šafárik University, Faculty of Medicine, Department of Histology and Embryology, Košice, Slovak Republic

Received March 1, 2004
Accepted May 1, 2004


1. Attaix D, Combaret L, Pouch MN, Taillandier D. Cellular control of ubiquitinproteasome- dependent proteolysis. J Anim Sci 2002; 80:E56–E63. <>
2. Burda J, Martin ME, Garcia A, Alcazar A, Fando JK, Salinas M. Phosphorylation of the alfa subunit of initiation factor 2 correlates with the inhibition of translation following transient cerebral ischemia in the rat. Biochem J 1994; 302:361–75. <> <PubMed>
3. Hu BR, Martone ME, Jones YZ, Liu CL. Protein aggregation after transient cerebral ischemia. J Neurosci 2000; 20(9):3191–9. <> <PubMed>
4. Ide T, Takada K, Qiu JH et al. Ubiquitin stress response in postischemic hippocampal neurons under nontolerant and tolerant conditions. J Cerebral Blood Flow Metabol 1999; 19:750–6. <>
5. Maršala J, Šulla J, Šanta M, Maršala M, Mechírová E, Jalč P. Early neurohistopathological changes of canine lumbosacral spinal cord in ischemia-reperfusioninduced damage. Neurosci Lett 1989; 106:83–8. <>
6. Matts RL, Hurst R, Xu Z. Denatured proteins inhibit translation in hemin-supplemented rabbit reticulocyte lysate by inducing the activation of the heme-regulated elF-2 alfa kinase. Biochemistry 1993; 32:7323–8. <>
7. Nauta WJH. Silver impregnation of degenerating axons. In: New research techniques of Neuroanatomy. Springfield: Thomas, 1957:17–26.
8. Peters JM. Proteasomes: protein degradation machines of the cell. Trends Biochem Sci 1994; 19:377–82. <>
9. Siesjö BR, Hu BR, Kristian T. Is the cell death pathway triggered by the mitochondrion or the endoplasmic reticulum? J Cereb Blood Flow Metab 1999; 19:19–26. <>
10. Schmidtová K, Kočišová M, Siroťáková M. Histochemical localization of NADPHd in neurons of the pheasant ileum. Acta Histochem 2002; 104(4):423–6. <>
11. Vanucci SJ, Mummery R, Hawkes RB, Rider CC, Beesley PW. Hypoxia-ischemia induces a rapid elevation of ubiquitin conjugates levels and ubiquitin immunoreactivity in the immature rat brain. J Cerebral Blod Flow Metab 1998; 18:376–85. <>
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