Acta Med. 2004, 47: 205-208

Acetylcholinesterase (AChE) – Positive Innervation of the Guinea-Pig Spleen

Katarína Schmidtováa, Mária Siroťákováb, Monika Kočišováa, Eva Mechírovác

aUniversity P. J. Šafárik, Faculty of Medicine, Department of Anatomy, Košice, Slovak Republic
bUniversity P. J. Šafárik, Faculty of Medicine, Institute of Experimental Medicine, Košice, Slovak Republic
cUniversity P. J. Šafárik, Faculty of Medicine, Department of Histology and Embryology, Košice, Slovak Republic

Received March 1, 2004
Accepted June 1, 2004

The presence and intraorgan distribution of the acetylcholinesterase (AChE)- positive nerve structures in the guinea-pig spleen were studied by means of the direct thiocholine method. Visualized AChE-positive nerve fibres entered the guinea-pig spleen at its hilum in the vicinity of the splenic artery branches and intra parenchyma were gradually distributed to form thicker periarterial nerves and also fine adventitial nerve plexus. In described topography the AChE-positive nerve fibres were identified in association with the central artery running through the white pulp. Some of the perivascular nerve fibres associated with the central artery extended away and passed into the periarterial lymphatic sheath (PALS) to reach the marginal zone and in continuation entered into the mantle zone of lymphatic follicles. Several AChE-positive nerve fibres were seen in the red pulp but less in the splenic capsule. We did not find any AChE – positive nerve cells in the guinea-pig spleen.


This work was supported by a VEGA grant No. 1/8294/01 Ministry of Education of Slovak Republic and by Internal grant No.19/2002/IG4 Faculty of Medicine P. J. Šafárik University, Košice.


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