The Multidrug Resistance and Apoptosis Evaluation in Acute Myeloid Leukemia Cells After the In Vitro Doxorubicin Treatment

Jankovičová, Karolína; Krejsek, Jan; Kopecký, Otakar; Voglová, Jaroslava; Škrabková, Zuzana; Novosad, Jakub

doi:10.14712/18059694.2018.88

Acta Medica > Archive > 2004, Vol. 47 > Issue 3 > The Multidrug Resistance and Apoptosis…

Acta Med. 2004, 47: 181-185

https://doi.org/10.14712/18059694.2018.88

The Multidrug Resistance and Apoptosis Evaluation in Acute Myeloid Leukemia Cells After the In Vitro Doxorubicin Treatment

Karolína Jankovičová^a, Jan Krejsek^a, Otakar Kopecký^a, Jaroslava Voglová^b, Zuzana Škrabková^c, Jakub Novosad^a

^aCharles University in Prague, Faculty of Medicine and University Hospital in Hradec Králové, Department of Clinical Immunology and Allergology, Hradec Králové, Czech Republic
^bCharles University in Prague, Faculty of Medicine and University Hospital in Hradec Králové, Department of Clinical Hematology, Hradec Králové, Czech Republic
^cCharles University in Prague, Faculty of Medicine and University Hospital in Hradec Králové, Department of Medical Biophysics, Hradec Králové, Czech Republic

Received April 1, 2004
Accepted May 1, 2004

The apoptosis failure in cytostatic treatment of haemoblastosis is one of the means of chemoresistance. We were interested in the relationship of the after-doxorubicin-treatment-AML cells apoptosis and the immunophenotype, selected clinical and laboratory parameters, and also the P-gp, MRP, LRP, Bcl-2, Bax proteins expression. All analysis were evaluated with the flow cytometry method. To detect apoptotic cells in the sample, we used three methods: annexin test, TUNEL (TdT-mediated dUTP nick end labeling) test, and caspase 8 detection. After the cell cultivation the statisticaly important increase of apoptotic cells in the culture was apparent. The relation between the AML blast in vitro reaction and some clinical parameters such as the age of patient, white blood cell count, and blast percentage was also observed.