Acta Med. 2004, 47: 119-123

Altered Intrahepatic Hematopoiesis in Neonates from Women with Pregnancy Induced Hypertension/Pre-Eclampsia

Demetrio Tamiolakisa, Ioannis Venizelosb, Maria Lambropoulouc, Anna Efthymiadouc, Vasiliki Arvanitidouc, Panagiotis Tsikourasd, Gerasimos Koutsougerase, George Chimonise, Demetrio Karamanidise, Sophia Barbagadakia, Sylva Nikolaidoua, Evagelia Seliniotakia, Panagiotis Boglouc, Nikolas Papadopoulosc

aGeneral Hospital of Chania, Department of Cytology, Greece
bIppokration Hospital of Salonica, Department of Pathology, Greece
cDemocritus University of Thrace, Department of Histology–Embryology, Greece
dDemocritus University of Thrace, Department of Obstetrics and Gynecology, Greece
eGeneral Hospital of Alexandroupolis, Department of Obstetrics and Gynecology, Greece

Received January 1, 2004
Accepted March 1, 2004

Aim: To detect whether preeclampsia influences neonatal intrahepatic hematopoiesis, given that an activation of fetal neutrophils and monocytes during the course of this disorder occurs. Experimental design: We examined liver samples from 10 neonates of hypertensive/preeclamptic women at 27 to 28 weeks of gestation delivered by a cessarian section. All neonates were placed in incubators but they all died within 24 hours due to immaturity. The control group comprised 10 fetuses of the same gestational age, after voluntary abortion due to a neural defect. Specific antibodies against CD34, glycophorin C, hemoglobins A and F, myeloperoxidase, CD61, CD68, terminal desoxynucleotidyl transferase and the pax-5/B-cell specific activator protein, were used in each sample. Results: Neonates from hypertensive/preeclamptic women, in comparison with controls, showed: a statistically significant reduction of erythropoiesis by 25% (p=0.015); a statistically significant increase of granulopoiesis (p=0.019); a statistically significant increase in the expression of CD68 positive cells of the monocytic lineage (p=0.017); a statistically significant increase in the expression of CD34 progenitor/stem positive cells (p=0.021). No statistically significant differences were observed in both examined groups, concerning megakaryopoiesis and B lymphopoiesis. Conclusions: Preeclampsia of pregnancy has an impact on neonatal intrahepatic hematopoiesis by increasing granulopoiesis, reducing erythropoiesis and triggering endothelial and stem cell activation. We suggest that these findings reflect a state of persistent inflammation and a loss of red blood cell production possibly contributing to the neonatal morbidity related to this disorder.


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