Acta Med. 2002, 45: 135-144
Time Dependent Appearance of Selected Apoptotic Markers and Usefulness of Their Detection In vitro
Many experiments have demonstrated that some cell lines are resistant to chemically induced apoptosis in vitro, and that apoptosis itself is far from being a homogenous phenomenon. Here we show that 10 μg/ml etoposide elicited only minor changes in Bowes human melanoma cells (temporary decrease in cell viability and proliferation, transient phospatidylserine externalization and caspase-3 activation), which weren’t clearly capable to start apoptotic pathway in the entire treated population. On the other hand, potassium chromate at concentration of 150 μg/ml executed cell death bearing some features of apoptosis (cell blebbing, caspase-3 activation and cytoskeletal changes) but lacking or showing weakly others (DNA fragmentation and phospatidylserine externalization). Our results suggest that in detecting apoptosis several faultproof detection systems are to be used to avoid misleading results and conclusions in each experimental setting.
Keywords
Melanoma, Caspase-3, Chromate, Apoptosis, Detection, Phospatidylserine, Etoposide, In vitro.
Funding
This work was supported by Ministry of Education Czech Republic Research Project FJ MSM 111500001.
References
Copyright
Published by the Karolinum Press. For permission to use please write to actamedica@lfhk.cuni.cz.
