Acta Med. 2001, 44: 7-13

Light-Induced Photoactivation of Hypericin Inhibits Cellular Growth in Pituitary Adenoma Cell Line AtT20/D16v-F2 (Hypericin Inhibits Cellular Growth of AtT20/D16v-F2)

Martina Marekováa, Jiřina Vávrováb, Doris Vokurkovác, Jaroslav Cermana

aCharles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biochemistry, Hradec Králové, Czech Republic
bPurkyně Military Medical Academy in Hradec Králové, Department of Radiobiology and Immunology, Hradec Králové, Czech Republic
cUniversity Teaching Hospital, Hradec Králové, Department of Clinical Immunology and Allergology, Hradec Králové, Czech Republic

Received September 1, 2000
Accepted January 1, 2001

Cultivation with 4-8 μmol/l hypericin (specific proteinkinase C inhibitor) activated by light induced high inhibition of the rate of HL-60 cell growth. When hypericin treated cells were not exposed to light growth inhibition was insignificant. Cultivation with light activated hypericin in concentration 16 μmol/l slightly inhibited growth rate of AtT20/D16v-F2 cells. AtT20/D16v-F2 cells did not proliferate in presence of light activated 32 μmol/l hypericin. Evidence of apoptosis was found in HL-60 cells treated with 1-8 μmol/l light activated hypericin, with maximum of apoptotic cells detected after 8 μmol/l light activated hypericin. Light activated hypericin induces both apoptosis and necrosis in dose and time dependent manners in HL-60 cells, but causes only necrosis in AtT20/D16v-F2 cells.


This work was supported by grants from Grant Agency of Charles University (GA UK 279/98), from Grant Agency of Ministry of Health (IGA-MZ: NB/6172-3) and in part by research project J13/98: 111500001.


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