Light-Induced Photoactivation of Hypericin Inhibits Cellular Growth in Pituitary Adenoma Cell Line AtT20/D16v-F2 (Hypericin Inhibits Cellular Growth of AtT20/D16v-F2)

Mareková, Martina; Vávrová, Jiřina; Vokurková, Doris; Cerman, Jaroslav

doi:10.14712/18059694.2019.80

Acta Medica > Archive > 2001, Vol. 44 > Issue 1 > Light-Induced Photoactivation of…

Acta Med. 2001, 44: 7-13

https://doi.org/10.14712/18059694.2019.80

Light-Induced Photoactivation of Hypericin Inhibits Cellular Growth in Pituitary Adenoma Cell Line AtT20/D16v-F2 (Hypericin Inhibits Cellular Growth of AtT20/D16v-F2)

Martina Mareková^a, Jiřina Vávrová^b, Doris Vokurková^c, Jaroslav Cerman^a

^aCharles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biochemistry, Hradec Králové, Czech Republic
^bPurkyně Military Medical Academy in Hradec Králové, Department of Radiobiology and Immunology, Hradec Králové, Czech Republic
^cUniversity Teaching Hospital, Hradec Králové, Department of Clinical Immunology and Allergology, Hradec Králové, Czech Republic

Received September 1, 2000
Accepted January 1, 2001

Cultivation with 4-8 μmol/l hypericin (specific proteinkinase C inhibitor) activated by light induced high inhibition of the rate of HL-60 cell growth. When hypericin treated cells were not exposed to light growth inhibition was insignificant. Cultivation with light activated hypericin in concentration 16 μmol/l slightly inhibited growth rate of AtT20/D16v-F2 cells. AtT20/D16v-F2 cells did not proliferate in presence of light activated 32 μmol/l hypericin. Evidence of apoptosis was found in HL-60 cells treated with 1-8 μmol/l light activated hypericin, with maximum of apoptotic cells detected after 8 μmol/l light activated hypericin. Light activated hypericin induces both apoptosis and necrosis in dose and time dependent manners in HL-60 cells, but causes only necrosis in AtT20/D16v-F2 cells.

Keywords

AtT20/D16v-F2, Hypericin, Apoptosis, Proteinkinase C.

Funding

This work was supported by grants from Grant Agency of Charles University (GA UK 279/98), from Grant Agency of Ministry of Health (IGA-MZ: NB/6172-3) and in part by research project J13/98: 111500001.